有氧运动对慢性间歇性缺氧大鼠血糖及胰岛素水平影响的氧化应激机制

Effects of aerobic exercise on blood glucose and insulin levels in chronic intermittent hypoxic rats and mechanism of oxidative stress

目的探讨有氧运动对慢性间歇性缺氧(CIH)大鼠血糖及胰岛素水平的影响机制,为CIH引起糖尿病的防治提供新思路。方法SD大鼠随机分为空白对照组、CIH对照组和CIH运动组,适应性饲养后对CIH对照组和CIH运动组进行CIH模型建立,模型建立后CIH运动组进行无负重游泳训练,4周后处死所有大鼠,检测各组大鼠总抗氧化能力(T-AOC)、活性氧(ROS)、丙二醛(MDA)、空腹血糖(FPG)、空腹胰岛素(FINS)水平。结果空白对照组、CIH对照组、CIH运动组大鼠ROS、MDA、T-AOC、FINS、FPG比较,差异均有统计学意义(F=4.60、5.03、4.87、4.52、6.42,P=0.021、0.015、0.017、0.022、0.006)。组间两两比较结果显示,与空白对照组比较,CIH对照组和CIH运动组大鼠ROS、MDA、FPG、FINS均升高,T-AOC均降低(均P<0.05);与CIH对照组比较,CIH有氧运动组大鼠ROS、MDA、FPG、FINS则降低、T-AOC升高(均P<0.05)。结论CIH通过激活氧化应激状态,血糖、胰岛素水平升高;有氧运动可降低氧化应激状态对血糖及胰岛素水平的影响。

Objective To investigate the effects of aerobic exercise on blood glucose and insulin levels in chronic intermittent hypoxic(CIH)rats and the underlying mechanisms,and to provide new insights for the prevention and treatment of diabetes caused by CIH.Methods SD rats were randomly divided into the blank control group,CIH control group and CIH exercise group.After adaptive feeding,a rat model of CIH was established.The CIH exercise group received non-weight bearing exercise training through swimming.After 4 weeks,all rats were sacrificed and levels of total antioxidant capacity(T-AOC),reactive oxygen species(ROS),malondialdehyde(MDA),fasting blood glucose(FPG)and fasting insulin(FINS)were measured.Results Compared with the blank control group and CIH control group,levels of ROS,MDA,FPG,T-AOC,FINS and FPG were significantly different in the CIH exercise group(F=4.60,5.03,4.87,4.52 and 6.42,P=0.021,0.015,0.017,0.022 and 0.006).Compared with the blank control group,levels of ROS,MDA,FPG and FINS increased and levels of T-AOC declined in the CIH control and exercise groups(all P<0.05).Compared with the CIH control group,levels of ROS,MDA,FPG and FINS decreased and T-AOC levels increased in the CIH exercise group(all P<0.05).Conclusions CIH increases blood glucose and insulin levels by activating the oxidative stress response.Aerobic exercise can reduce the impact of oxidative stress on blood glucose and insulin levels.