C-Kit、父系表达基因10在原发性肝癌患者中的表达及与临床特征和预后的关系

Expression of C-Kit and paternally expressed gene 10 in patients with primary liver cancer and their relationship with clinical characteristics and prognosis

目的探讨C-Kit、父系表达基因10(PEG10)在原发性肝癌(PLC)患者中的表达及与临床特征和预后的关系。方法收集PLC患者的PLC组织90例及癌旁正常组织90例,采用免疫组织化学染色法检测C-Kit和PEG10的表达情况。比较PLC组织和癌旁正常组织中C-Kit和PEG10蛋白的表达情况,比较不同临床特征PLC患者PLC组织中C-Kit和PEG10蛋白的表达情况。采用Logistic回归模型分析PLC患者预后的影响因素。结果PLC组织中C-Kit和PEG10蛋白的高表达率均明显高于癌旁正常组织,差异均有统计学意义(P﹤0.01)。临床分期为Ⅲ~Ⅳ期、低分化、甲胎蛋白(AFP)水平﹥200 ng/ml的PLC患者PLC组织中C-Kit和PEG10蛋白高表达率分别明显高于临床分期为Ⅰ~Ⅱ期、中高分化、AFP水平≤200 ng/ml的患者,差异均有统计学意义(P﹤0.01)。随访3年,90例PLC患者的3年生存率为51.11%(46/90)。单因素分析结果显示,临床分期为Ⅲ~Ⅳ期、低分化、预后评分﹤7分、C-Kit蛋白高表达、PEG10蛋白高表达、AFP水平﹥200 ng/ml的PLC患者的3年生存率分别明显低于临床分期为Ⅰ~Ⅱ期、中高分化、预后评分≥7分、C-Kit蛋白低表达、PEG10蛋白低表达、AFP水平≤200 ng/ml的患者,差异均有统计学意义(P﹤0.01)。多因素Logistic回归分析结果显示,临床分期为Ⅲ~Ⅳ期、低分化、预后评分﹤7分、C-Kit蛋白高表达和PEG10蛋白高表达均是PLC患者预后的独立危险因素(P﹤0.05)。结论C-Kit和PEG10在PLC组织中高表达,其表达情况与患者的临床特征及预后有关,C-Kit和PEG10蛋白有可能作为理想的PLC预后标志物。

Objective To investigate the expression of C-Kit and paternally expressed gene 10(PEG 10)in patients with primary liver cancer(PLC)and their relationship with clinical characteristics and prognosis.Method A total of 90 PLC patients were enrolled,and their PLC tissues and paracancerous normal tissues were collected.The expression levels of C-Kit and PEG 10 were detected by the immunohistochemistry assay.The expressions of C-Kit and PEG 10 in PLC tissues and paracancerous normal tissues were compared,and the expressions of both indicators in PLC patients with different clinical characteristics were also compared.The prognostic factors of PLC patients were analyzed by the Logistic regression model.Result The high expression rates of C-Kit and PEG 10 protein in PLC tissues were significantly higher than those in paracancerous normal tissues,the differences were statistically significant(P<0.01).The high expression rates of C-Kit and PEG 10 protein in PLC tissues from patients with clinical stageⅢ-Ⅳ,poor differentiation,orα-fetoprotein(AFP)level>200 ng/ml were significantly higher than those of patients with clinical stageⅠ-Ⅱ,moderate to high differentiation,or AFP level≤200 ng/ml,and the differences were statistically significant(P<0.01).The patients were followed up for 3 years,and the 3-year overall survival of the 90 PLC patients was 51.11%(46/90).The results of the univariate analysis indicated that the 3-year overall survival of PLC patients with clinical stageⅢ-Ⅳ,poor differentiation,prognostic score<7,C-Kit protein high expression,PEG 10 protein high expression,or AFP level>200 ng/ml were significantly lower than those of patients with clinical stageⅠ-Ⅱ,moderate to high differentiation,prognostic score≥7,C-Kit protein low expression,PEG 10 protein low expression,or AFP level≤200 ng/ml,the differences were statistically significant(P<0.01).The results of multivariate Logistic regression analysis indicated that clinical stageⅢ-Ⅳ,poor differentiation,prognostic score<7,high expression of C-Kit and PEG 10 protein were all independent risk factors for the prognosis of PLC patients(P<0.05).Conclusion Both C-Kit and PEG 10 are up-regulation in PLC tissues,and their expression levels may be related to the clinical characteristics and prognosis of PLC patients.The two targets could be used as ideal prognostic markers for PLC evaluation.