miR-129-5p表达量与胃癌患者临床病理特征的关系分析

Relationship between the expression of miR-129-5p and clinicopathological features of gastric cancer

目的探讨miR-129-5p表达量与胃癌患者临床病理特征的关系。方法采用回顾性研究方法,选取2016年12月至2020年12月在蚌埠医学院第一附属医院治疗的胃癌患者95例,同时选取癌旁组织作为对照(距癌边缘>5 cm)。采用逆转录-聚合酶链反应(RT-PCR)检测组织miR-129-5p表达,比较不同病理特征胃癌组织miR-129-5p表达,以及不同miR-129-5p表达患者无进展生存时间差异。结果胃癌组织miR-129-5p相对表达量为0.522±0.101,明显低于癌旁组织(1.722±0.322),差异有统计学意义(P<0.05);肿瘤直径≥5 cm、有淋巴结转移、TNM分期Ⅲ期组织miR-129-5p相对表达量分别为0.431±0.105、0.443±0.105和0.430±0.101,明显低于肿瘤直径<5 cm(0.566±0.103)、无淋巴结转移(0.599±0.102)、TNM分期Ⅰ~Ⅱ期组织(0.592±0.103),差异均有统计学意义(P<0.05);miR-129-5p相对表达量与胃癌患者性别、年龄、分化程度无明显关系(P>0.05);miR-129-5p低表达患者无进展生存时间为25个月(95%CI:22.61~27.39),明显短于miR-129-5p高表达患者(P<0.05);Cox比例风险回归,结果显示:TNM分期、淋巴结转移和miR-129-5p是胃癌患者预后的影响因素(HR=2.779、1.667和0.587,P<0.05)。结论胃癌组织miR-129-5p表达下调,与淋巴结转移、肿瘤直径及TNM分期及预后有关,值得进一步研究。

Objective To investigate the relationship between the expression of miR-129-5p and clinicopathological features of gastric cancer.Methods Ninety-five cases of gastric cancer patients treated in the First Affiliated Hospital of Bengbu Medical College from December 2016 to December 2020 were retrospectively selected,and the adjacent tissues(>5 cm away from the edge of cancer)were selected as control,the expression of mir-129-5p was detected by reverse transcription-polymerase chain reaction(RT-PCR).The expression of miR-129-5p in gastric cancer tissues with different pathological characteristics was compared,and the progression free survival time of patients with different miR-129-5p expression was compared.Results The relative expression of miR-129-5p in gastric cancer tissues was 0.522±0.101,which was significantly lower than that in adjacent tissues(1.722±0.322),the difference was statistically significant(P<0.05).The relative expression levels of miR-129-5p in tumor diameter≥5 cm,lymph node metastasis and TNM stageⅢtissues were 0.431±0.105,0.443±0.105 and 0.430±0.101,which were significantly lower than those in tumor diameter<5 cm(0.566±0.103),no lymph node metastasis(0.599±0.102)and TNM stageⅠ-Ⅱtissues(0.592±0.103),the differences were statistically significant(P<0.05);The relative expression of miR-129-5p had no significant relationship with gender,age and differentiation degree of gastric cancer patients(P>0.05);The progression free survival time of patients with low expression of miR-129-5p was 25 months(95%CI:22.61-27.39),which was significantly shorter than that of patients with high expression of miR-129-5p(P<0.05);Cox proportional hazards regression showed that TNM stage,lymph node metastasis and miR-129-5p were the prognostic factors of gastric cancer(HR=2.779,1.667 and 0.587,P<0.05).Conclusion The expression of miR-129-5p is down-regulated in gastric cancer tissues,is related to lymph node metastasis,tumor diameter,TNM stage and prognosis,which deserves further study.