牙周膜干细胞BMP-2-PSH复合膜修复新西兰兔牙槽骨缺损

Periodontal Ligament Stem Cell BMP-2-PSH Composite Membrane in Repairing Alveolar Bone Defect in New Zealand Rabbits

目的拟构建新西兰兔牙周缺损模型,确定hPDLSCs-BMP-2-PSH膜的生物安全性和其体内成骨修复作用。方法在确定BMP-2-PSH膜没有细胞毒性后将培养好的hPDLSCs细胞膜片复合至BMP-2-PSH膜上。分别在每只新西兰兔下颌左中切牙牙槽骨缺损内植入hPDLSCs/PSH复合膜作为实验组,右中切牙牙槽骨缺损内植入对照性多孔纤维膜材料作为对照组(或不植入任何材料作为对照),然后全瓣复位,缝合。术后即刻和每3周(连续观察12周)进行影像学(CT)检查,观察骨缺损愈合情况;同时,分别在术后第4、8、12周观察PDLSCs/BMP-2双膜材料的吸收度和各组样本新骨形成量和新骨形态。结果不同浓度PSH膜和BMP-2-PSH膜浸提液对hPDLSCs细胞不具有细胞毒性,hPDLSCs细胞能够成功复合到BMP-2-PSH膜上;CBCT及组织形态学分析显示,hPDLSCs/PSH复合膜能够有效促进牙槽骨缺损修复再生。结论hPDLSCs/PSH复合膜具有良好生物安全性,有利于修复新西兰兔牙周缺损。

Objective It is planned to build a New Zealand rabbit periodontal defect model and determine the biological safety of hPDLSCs-BMP-2-PSH membrane and its in vivo osteogenic repair effect.Methods After confirming that the BMP-2-PSH membrane was not cytotoxic,the cultured hPDLSCs cell membrane was compounded to the BMP-2-PSH membrane.Respectively,HPDLSCs/PSH composite membrane was implanted into the alveolar bone defect of mandibular left incisor in each New Zealand rabbit as the experimental group,and the controlled porous fiber membrane material was implanted into alveolar bone defect of the right central incisor as the control group(or not implanted any material was used as a control),then the whole flap was reset and sutured.Imaging(CT)examination was performed immediately after the surgery and every 3 weeks(observed continuously for 12 weeks)to observe the healing of bone defects;meanwhile,the absorbance of PDLSCs/BMP-2 double membrane materials,the amount of new bone formation and new bone morphology of samples in each group were observed at 4,8 and 12 weeks after the surgery.Results Different concentrations of PSH membrane and BMP-2-PSH membrane extracts were not cytotoxic to hPDLSCs cells,hPDLSCs cells could be successfully compounded to BMP-2-PSH membranes;The analysis of CBCT and histomorphology showed that hPDLSCs/PSH composite membranes can effectively promote the repair and regeneration of alveolar bone defects.Conclusion The hPDLSCs/PSH composite membrane has the good biological safety and is beneficial to repair periodontal defects of New Zealand rabbits.