MPTP诱导树鼩帕金森病模型的脑电图描记与分析

The Electroencephalogram and Analysis of MPTP-Induced Tree Shrew Model for Parkinson's Disease

目的研究树鼩帕金森病(PD)模型的皮层脑电图和脑电功率谱、地形图特征。方法选取普通级成年滇缅树鼩10只,随机分为模型组(n=5)和对照组(n=5)。采用1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)2 mg/kg肌肉注射建立树鼩PD模型,对照组注射等体积0.9%氯化钠溶液,连续15 d。停药1月后,采用EEG技术对树鼩PD模型进行皮层脑电图描记与功率谱、地形图分析。结果成功建立树鼩PD模型。正常对照组树鼩大脑半球额部、顶部、枕部等各部位脑电波的频率、波幅、波形、相位无明显差异和特异性。脑电波各部位α波占主导,波幅差<30%;背景为散在δ、θ波;脑电功率谱直方图,两半球各频带功率值分布对称;地形图双侧六频带为低功率频谱,其功率值<100μv^(2)/Hz。树鼩PD模型组记录到棘波、尖波等异常波形呈节律性发放,主要表现为α波左右不对称,异常波形主要表现在额部和顶部,部分出现高振幅慢波δ、θ;脑电功率谱主要表现为α波相位不同步,各频带功率谱直方图分布不对称;树鼩PD模型组地形图δ频段功率平均值>1000μv^(2)/Hz,为低频高功率。δ、θ、α1、α2频带功率值与对照组相比显著性增高。结论树鼩PD模型脑电波形图、脑电频谱直方图及脑电地形图较正常对照组具有显著的特异性,可为PD模型神经电生理研究提供参考。

Objective To study the characteristics of cortical electroencephalogram(EEG),power spectrum and mapping in tree shrew model for Parkinson’s disease(PD).Methods Ten common-level Burmese adult tree shrews were selected and randomly divided into a model group(n=5)and a control group(n=5).A PD model of tree shrew was produced by intramuscular injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP),with a MPTP dose of 2 mg/kg.The control group was injected with an equal volume of 0.9%sodium chloride solution continuously for 15 days.One month after the drug withdrawal,EEG technology was used to perform the cortical EEG tracing,power spectrum and topographic analysis of the tree shrew PD model.Results The MPTPInduced Tree Shrew Model for PD was established successfully.In the normal control group,there was no significant difference and specificity in frequency,amplitude,waveform and phase of EEG in the frontal,parietal and occipital parts of the cerebral hemisphere,αwave was dominant in the waves recorded in each part of the tree shrews brain and amplitude difference was less than 30%,withδandθwaves being scattered;The histogram of EEG power spectrum was basically symmetrical on both sides;The power value of each frequency band was distributed symmetrically in the two hemispheres and the bilateral six bands in the EEG mapping was low power and its power value was less than 100μv^(2)/Hz.In the tree shrew PD model group,spikes,sharp waves and other abnormal waves were recorded,showing rhythmic emission.The mainly manifestedαwave was asymmetry,the abnormal waveforms were mainly in the frontal and parietal areas,and the high amplitude slow wavesδ、θalso appeared in some areas;The main features of EEG power spectrum were as follows:αwave phase was not synchronous,and the histogram distribution of power spectrum in each frequency band was asymmetric;Theδfrequency band in the EEG mapping was low frequency and high power and the average power was more than 1000μv^(2)/Hz.Compared with the control group,the frequency band power values ofδ、θ、α1andα2 were significantly higher than those of the control group.Conclusion Compared with the normal control group,the EEG waveforms,EEG spectrum histogram and EEG mapping in the tree shrew PD model all show the significant characteristics,proving that they can provide references for the study of neuroelectrophysiology in PD model.