核磁共振在评价超短波治疗对兔膝关节损伤模型关节功能恢复中的价值

Values of MRI in evaluating the recovery of joint function of rabbit knee joint injury model by ultrashort wave treatment

目的探讨核磁共振在评价超短波治疗对兔膝关节损伤模型关节功能恢复中的价值。方法纯种雄性新西兰大白兔24只按随机数字表法分为空白组、模型组与超短波组,每组各8只。空白组不造模不治疗,模型组造模成功后不治疗,超短波组造模成功后第2天予以超短波治疗,连续治疗28 d。分别于造模前1 d和治疗28 d后测定大白兔的右膝关节活动范围;治疗28 d后,采用黄嘌呤氧化酶法测定血清超氧化物歧化酶(SOD)含量,硫代巴比妥法检测血清丙二醛(MDA)含量;TUNEL法检测软骨细胞凋亡指数;核磁共振成像评定各组兔膝关节功能恢复情况。结果实验前,3组大白兔右膝关节活动度对比,差异无统计学意义(P>0.05);治疗28 d后,模型组与超短波组的膝关节活动度为(67.09±10.88)°、(98.02±11.38)°,均低于空白组[(143.29±17.02)°],超短波组膝关节活动度高于模型组,差异均有统计学意义(P<0.05)。治疗28 d后,模型组与超短波组的血清SOD含量为(78.98±8.29)NU/mL、(94.92±9.11)NU/mL,低于空白组[(111.32±10.38)NU/mL],MDA含量为(6.76±0.33)nmol/mL、(4.44±0.22)nmol/mL,高于空白组[(3.87±0.18)nmol/mL],超短波组大白兔血清SOD含量显著高于模型组,MDA含量显著低于模型组,差异均有统计学意义(P<0.05)。治疗28 d后,模型组与超短波组大白兔的软骨细胞凋亡指数分别为(8.20±0.33)%、(4.10±0.72)%,高于空白组的(0.98±0.14)%,超短波组软骨细胞凋亡指数低于模型组,差异均有统计学意义(P<0.05)。模型组与超短波组的膝关节T1弛豫时间与磁化传递率分别为(702.58±23.77)ms、(567.09±31.72)ms;(0.65±0.09)%、(0.45±0.08)%,高于空白组[(443.59±33.29)ms;(0.32±0.08)%],而超短波组膝关节T1弛豫时间与磁化传递率显著低于模型组,差异均有统计学意义(P<0.05)。结论超短波治疗在兔膝关节损伤模型的应用能改善核磁共振成像指标,降低软骨细胞凋亡指数,提高SOD含量与降低MDA含量,从而促进关节功能恢复。

Objective To explore the values of MRI in the evaluation of ultrashort wave therapy in the recovery of joint function of rabbit knee injury models.Methods Pure-bred male New Zealand white 24 rabbits were equally divided into blank group,model group and ultrashort wave group accorded to the random number table method,8 rabbits in each group.The blank group were not modeled and treated,the model group were treated after successful modeling,the ultrashort wave group were received ultrashort wave treatment on the 2nd day after the successful modeling,and continued treatment for 28 days.The range of motion of the right knee joint of the white rabbits was measured before and after 1 day of modeling and 28 days after treatment;After 28 days of treatment,the xanthine oxidase method was used to measure the serum superoxide dismutase(SOD)content,and the thiobarbital method was used to measure the serum malondialdehyde(MDA)content;TUNEL method to detect chondrocyte apoptosis index;MRI was used to evaluate the functional recovery of the rabbits'knee joints in each group.Results Before the experiment,there was no significant difference in the range of motion of the right knee joint between the three groups of white rabbits(P>0.05);after 28 days of treatment,the range of motion of the knee joint between the model group and the ultrashort wave group was(67.09±10.88)°,(98.02)±11.38)°,which were all lower than those in the blank group[(143.29±17.02)°],the range of motion of the knee in the ultrashort wave group was higher than that of the model group,and the differences were statistically significant(P<0.05).After 28 days of treatment,the serum SOD levels of the model group and the ultrashort wave group were(78.98±8.29)NU/mL and(94.92±9.11)NU/mL,which were lower than those in the blank group[(111.32±10.38)NU/mL],MDA content were(6.76±0.33)nmol/mL and(4.44±0.22)nmol/mL,which were higher than those in the blank group[(3.87±0.18)nmol/mL].The serum SOD content in the ultrashort wave group was significantly higher than that in the model group,and the MDA content was significant lower than in the model group,the differences were statistically significant(P<0.05).After 28 days of treatment,the apoptotic index of chondrocytes in the model group and the ultrashort wave group were(8.20±0.33)%and(4.10±0.72)%,respectively,which were higher than those in the blank group(0.98±0.14)%.the apoptotic index of chondrocytes in the ultrashort wave group was lower than that in the model group,and the difference was statistically significant(P<0.05).The knee joint T1 relaxation time and magnetization transfer rate of the model group and the ultrashort wave group were(702.58±23.77)ms,(567.09±31.72)ms;(0.65±0.09)%,(0.45±0.08)%,respectively,which were higher than those in the blank group[(443.59±33.29)ms;(0.32±0.08)%],while the ultrashort wave group knee joint T1 relaxation time and magnetization transfer rate were significantly lower than the model group,the differences were statistically significant(P<0.05).Conclusion The application of ultrashort wave therapy in the rabbit knee joint injury model can improve the MRI index,reduce the chondrocyte apoptosis index,increase the SOD content and reduce the MDA content,thereby promote the recovery of joint function.