生物信息学探究lncRNA CASC9在肝细胞癌中的功能及作用机制

Bioinformatics study on the function and mechanism of lncRNA CASC9 in hepatocellular carcinoma

目的探讨肝细胞癌(HCC)组织中长链非编码RNA(lncRNA)肿瘤易感性候选基因9(CASC9)的表达及其与临床预后的关系,并对CASC9靶基因进行系统的生物信息学分析。方法使用生物信息分析方法了解CASC9的基本信息与生物学功能;使用TCGA数据库数据,比较HCC组织和癌旁正常组织中CASC9表达量,并分析其与HCC预后的相关性;使用lnCAR数据库和Metascape工具对CASC9的靶基因进行预测及功能富集分析,并筛选出CASC9相互作用的miRNA和关键靶基因。结果与癌旁正常组织相比,CASC9在HCC组织中呈高表达(P<0.05)。生存分析显示,HCC组织中CASC9高表达与HCC患者的不良预后显著相关(P<0.05)。KEGG分析显示,CASC9与脂肪酸代谢、嘌呤代谢、MAPK信号通路、Jak-STAT信号通路、细胞周期等信号通路相关。GO富集分析结果显示,CASC9与细胞核分裂、细胞周期调控、细胞周期检查点等多种途经相关。结论CASC9可作为一种原癌基因参与HCC的发生、发展,并具有成为HCC诊断标志物、预后指标及治疗靶点的潜在价值。

Objective To investigate the expression of long non-coding RNA(lncRNA)cancer susceptibility candidate 9(CASC9)in hepatocellular carcinoma(HCC)tissues and its relationship with clinical prognosis,and to conduct systematically bioinformatics analysis of CASC9 target genes.Methods The basic information and biological function of CASC9 were analyzed by bioinformatics;the expression of CASC9 in HCC tissues and normal tissues adjacent to cancer was compared using TCGA database data,and the correlation with prognosis of HCC was analyzed.LnCAR database and Metascape tools were used to predict and enrich the target genes of CASC9,and miRNA and key target genes of CASC9 interaction were screened.Results Compared with normal tissues adjacent to cancer,CASC9 was highly expressed in HCC tissues(P<0.05).Survival analysis showed that high expression of CASC9 in HCC tissues was significantly correlated with poor prognosis of HCC patients(P<0.05).KEGG analysis showed that CASC9 was related to fatty acid metabolism,purine metabolism,MAPK signaling pathway,Jak-STAT signaling pathway,cell cycle and other signaling pathways.The results of GO enrichment analysis showed that CASC9 was related to multiple pathways such as cell nuclear division,cell cycle regulation,and cell cycle checkpoints.Conclusion CASC9 can be used as a proto-oncogene to participate in the occurrence and development of HCC,and has the potential value of becoming a diagnostic marker,prognostic indicator and treatment target of HCC.