2017-2019年重庆市自制抗酸染色标本室间质量评价结果分析

Analysis of the results of the external quality assessment of the homemade acid-fast staining specimens in Chongqing from 2017 to 2019

目的分析重庆市2017-2019年自制抗酸染色标本室间质量评价(EQA)结果,了解目前实验室人员的染色能力和读片能力,为提高该市抗酸染色显微镜检查质量寻找依据。方法重庆市临床检验中心发放自制的抗酸染色标本,收集参加抗酸染色显微镜检查EQA的各实验室结果,对结果进行统计分析。结果2017-2019年参加重庆市抗酸染色显微镜检查EQA的实验室数从2017年的58家增加至2019年的88家,合格率从2017年的96.6%增加至2019年的97.7%。5次抗酸染色显微镜检查EQA,阴性标本符合率97.8%,阳性标本符合率98.1%,总体符合率97.9%。结论通过自制标本开展抗酸染色显微镜检查EQA工作,实验室对抗酸染色显微镜检查质量控制越来越重视,抗酸染色显微镜检查质量有明显改进,推动了抗酸染色显微镜检查结果的准确性、可比性,为临床诊疗提供快速可靠的依据。

Objective To analyze the results of external quality assessment(EQA)of self-made acid fast staining specimens in Chongqing from 2017 to 2019,to understand the dyeing ability and smear reading ability of laboratory personnel,and to find the basis for improving the quality of acid-fast staining microscopy in Chongqing.Methods The self-made acid-fast staining specimens were distributed by Chongqing Center for Clinical Laboratory,and the results of each laboratory participating in acid-fast staining microscope examination of EQA were collected and statistically analyzed.Results From 2017 to 2019,the number of laboratories for EQA inspection by acid-fast staining microscopy in Chongqing increased from 58 in 2017 to 88 in 2019,and the qualified rate increased from 96.6%in 2017 to 97.7%in 2019.Five times of acid-fast staining microscopic examinations of EQA,the coincidence rate of negative specimens was 97.8%,the coincidence rate of positive specimens was 98.1%,and the overall coincidence rate was 97.9%.Conclusion Through self-made specimens to carry out the EQA work of acid-fast staining microscopy,the laboratory pays more and more attention to the quality control of acid-fast staining microscopy,and the quality of acid-fast staining microscopy has been significantly improved,which promotes the accuracy and comparability of acid-fast staining microscopy results,and provides fast and reliable basis for clinical diagnosis and treatment.