失眠患者血清钙调蛋白及GABA/Glu水平检测及其意义

Detection and significance of the level of serum calmodulin and GABA/Glu in insomnia patients

目的检测血清钙调蛋白(CaM)及γ-氨基丁酸(GABA)、谷氨酸(Glu)、GABA/Glu在失眠患者血清中的水平,探讨其与失眠的相关性。方法采用病例对照研究,收集符合入选标准的119例失眠患者作为研究组,收集122例健康体检者作为健康对照组。运用酶联免疫吸附法(ELISA法)检测所有入组者血清CaM、GABA、Glu、GABA/Glu水平,并分析CaM水平与GABA/Glu的相关性。结果(1)失眠组患者与对健康照组相比,血清CaM、GABA、GABA/Glu水平降低,Glu水平升高,有统计学意义(P<0.05)。(2)失眠患者血清CaM水平与GABA/Glu水平呈正相关(r=0.475,P<0.01)。结论CaM下调增加失眠的风险,其机制可能与下调GABA/Glu水平有关。

Objective We detected the level of serum calmodulin,γ-aminobutyric acid(GABA),glutamic acid(Glu)and GABA/Glu in insomnia patients,explored their association with insomnia.Methods The case-control study was used.This study comprised 119 insomnia patients and 122 healthy control subjects.Enzyme-linked immunosorbent assay(ELISA)was utilized to measure the level of serum CaM,GABA,Glu and GABA/Glu.The correlation between the level of serum CaM and GABA/Glu was performed by correlation analyze.Results Our results suggested the level of serum CaM,GABA,GABA/Glu were significantly lower,and the level of serum Glu was significantly higher in patients with insomnia compared to the control group(P<0.05).The level of serum CaM was positively correlated with GABA/Glu in insomnia patients(r=0.475,P<0.05).Conclusion CaM downregulation increases the risk of insomnia,and the mechanism may be related to the downregulation of GABA/Glu level.