摘要
目的评估补肾祛寒治尪汤(简称"治尪汤")联合甲氨蝶呤(MTX)对大鼠胶原诱导关节炎(CIA)模型的作用及高频超声在大鼠关节炎评估中的应用。方法 SD大鼠随机分为正常组、模型组、MTX组、治尪汤低剂量联合组、治尪汤高剂量联合组;除正常组外,其余大鼠分别于第1、7天尾部注射浓度为2 mg/mL的Ⅱ胶原乳剂50μL建立CIA模型;第8天开始给药21 d,检测足趾厚度,进行关节炎指数评分,并采用高频超声评估大鼠关节滑膜炎。结果造模后,各组大鼠足趾厚度较正常组明显增厚(P<0.01),第19天治尪汤高剂量联合组大鼠足趾厚度小于模型组(P<0.05);AI评分方面,第13天治尪汤高剂量联合组大鼠AI评分小于模型组(P<0.01);各组与正常组比较滑膜显著增厚,治尪汤低、高剂量联合组与模型组比较滑膜厚度显著降低,治尪汤高剂量联合组较MTX组滑膜厚度显著降低(P<0.05,P<0.01)。结论高频超声适用于大鼠CIA模型滑膜炎的评估,敏感性优于足趾厚度检测和AI评分,高剂量治尪汤与MTX联用对改善CIA大鼠关节炎有协同增效作用。
Objective To access the anti-inflammatory effect of kidney-supplementing cold-dispelling Zhiwang Decoction(Zhiwang Decoction) combined with methotrexate(MTX) on collagen-induced arthritis(CIA) model rats and the performance of high frequency ultrasound in the evaluation of arthritis in rats. Methods SD rats were randomly divided into 5 groups: normal group, model group, MTX group, low-dose of Zhiwang Decoction combined group and high-dose of Zhiwang Decoction combined group. Except for the normal group, rats of the other groups were injected in the tail with 50 μL collagen Ⅱ emulsion of 2 mg/mL on the 7 th and 14 th day of rearing respectively. A total of 21 days of administration began on the 8 th day. Toe thickness was measured, arthritis index(AI) was scored and ankle synovitis was evaluated by high-frequency gray-scale ultrasound in rats. Results After modeling, the toe thickness of each group was significantly thicker than that of the normal group(P<0.01). on the 19 th day, the toe thickness of the high-dose combined group was thinner than that of the model group(P<0.05). In terms of AI score, on the 13 th day, the AI score of the high-dose combined group was lower than that of the model group(P<0.01). Evaluated by the ultrasound, the synovial thickness of each group was significantly thicker than that of the normal group, the synovium of the low-dose and high-dose groups was significantly thinner than that of the model group, and the synovium of the high-dose group was significantly thinner than that of the MTX group(P<0.05, p < 0.01). Conclusion High-frequency ultrasound is suitable for the evaluation of synovitis in CIA model rats,and its sensitivity is better than toe thickness and AI score. High-dose Zhiwang Decoction combined with MTX has a synergistic effect on improving arthritis in CIA rats.
作者
马茹
陈冬雪
鄢泽然
陈光耀
徐愿
刘振权
MA Ru;CHEN Dongxue;YAN Zeran;CHEN Guangyao;XU Yuan;LIU Zhenquan(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100029,China;Inner Mongolia Medical University,Huhehaote,Inner Mongolia 010110,China;Beijing Key Laboratory of Immune Inflammatory Diseases,Rheumatology Department of TCM,China-Japan Friendship Hospital,Beijing 100029,China;National Institute for Traditional Chinese Medicine Constitution and Preventive Medicine,Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《上海中医药杂志》
2021年第4期85-91,共7页
Shanghai Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81603588)。
关键词
补肾袪寒治尪汤
类风湿关节炎
大鼠胶原诱导关节炎模型
高频超声
灰阶滑膜炎
kidney-supplementing cold-dispelling Zhiwang Decoction
rheumatoid arthritis
collagen-induced arthritis rat model
high frequency ultrasound
gray scale synovitis