人工湿地植物根际降解β-HCH细菌筛选及鉴定

Screening and Identification of Bacteria β-HCH Rhizosphere Degradation in Constructed Wetland Plants

从可稳定处理β-HCH的人工湿地模型取植物根际筛选降解菌,以提高人工湿地对β-HCH的降解效果。最终筛选出3株细菌,可辅助对β-HCH进行生物降解。3株菌经16sRNA鉴定分属于苍白杆菌属(Ochrobactrum)和假单胞菌属(Pseudomonas)。结果表明:实验室条件下,3株菌在30℃、200 r·min^(-1)摇床培养5 d后,对10μg·L^(-1)β-HCH的平均去除率分别达66.31%、67.38%和62.68%,对100μg·L^(-1)β-HCH的平均去除率分别达61.61%、64.07%和59.26%。3株菌在对数生长期平均可降解44.84%的β-HCH。随着β-HCH质量浓度的升高,菌株生长受到抑制。菌株的投加量对β-HCH降解效果影响不明显,复合菌群对β-HCH降解效果不理想。

The plant rhizosphere peat water mixtures were taken from artificial wetland models that can adequately degrade β-HCH,and degradative bacteria were screened to further improve the degradation effect of artificial wetlands on β-HCH. Three strains of bacteria were finally screened,which could assist the biodegradation of β-HCH.Three strains were identified by 16 sRNA and belonged to the genera Ochrobactrum and Pseudomonas. The results show that the average removal rates of β-HCH from the three strains under laboratory conditions reach 66.31%,67.38% and 62.68% respectively for 10 μg·L-1β-HCH at 30 ℃ in a shaker at 200 r·min-1 for 5 days,and 61.61%,64.07%,59.26% respectively for 100 μg·L-1β-HCH.The three strains degrade 44.84% β-HCH on average in logarithmic growth phase. Strain growth is inhibited with increasing concentrations of β-HCH.The dosing amount of the strains does not significantly affect their efficacy in degrading β-HCH,and the composite flora has an unsatisfactory effect on β-HCH degradation.