填料组成及微观结构对胰高血糖素样肽-1的分离效果研究

Effect of composition and microstructure of packings on separation of glucagon-like peptide-1

胰高血糖素样肽-1(GLP-1)在细胞构建过程中产生信号肽和N-2、N-22截短型等多种杂质,分离纯化难度大。以聚甲基丙烯酸酯(PMMA)、聚苯乙烯-二乙烯基苯(PS-DVB)和交联琼脂糖(AG)3种不同基质(matrix)的阴离子交换填料为研究对象,探讨填料基质、表面微观结构与层析分离效果之间的关系。研究结果表明,PMMA强阴离子交换填料对分离GLP-1具有高选择性;粒径均匀度、机械强度以及表面孔径对样品分离效果具有很大影响。

During cell construction process,glucagon-like peptide-1(GLP-1)generates a variety of impurities such as signal peptides and N-2,N-22 truncated types,which is difficult to separate and purify.Three anion exchange packings,including polymethyl methacrylate(PMMA),polystyrene-divinylbenzene(PS-DVB)and cross-linked agarose(AG),are used as research objects.Relationships among packings matrix,surface microstructure and separation effect are discussed.Results show that PMMA packings exhibit a high selectivity for the separation of GLP-1.Particle size uniformity,mechanical strength and surface pore diameter have great influences on the separation effect.