高效液相色谱-电喷雾质谱分析高粱中多酚种类

Analysis of polyphenols in sorghum by high performance liquid chromatography-electrospray mass spectrometry

建立了高效液相色谱-电喷雾-四级杆飞行时间质谱(HPLC-ESI-Q-TOF)分析高粱多酚的方法。色谱条件为:流动相A为体积分数为0.1%甲酸的H_(2)O,流动相B为体积分数为0.1%甲酸的乙腈,流动相梯度A 0~20 min 85%~40%、20~23 min 40%~10%、23~28 min 10%~10%、28~30 min 10%~85%、30~37 min 85%~85%;流速0.2 mL/min,进样量5μL,柱温30℃。质谱条件为:负离子模式下,毛细管电压2.6 kV,干燥气温度180℃,干燥气流量6 L/min,辅助气压力0.08 MPa,质荷比采集范围100~1200 m/z,碰撞能量为35 eV。对5种高粱所含的多酚类物质进行分析,共鉴定出20种高粱多酚,包括10种酚酸类物质、3种原花青素、7种酚酸类衍生物。

A high performance liquid chromatography-electrospray-quadrupole time-of-flight mass spectrometry(HPLC-ESI-Q-TOF)method is established for the analysis of sorghum polyphenols.Chromatographic conditions are as follows:mobile phase A is 0.1%formic acid in H_(2)O,mobile phase B is 0.1%formic acid in acetonitrile,mobile phase gradient A is 0-20 min 85%-40%;20-23 min 40%-10%;23-28 min 10%-10%;28-30 min 10%-85%;30-37 min 85%-85%;flow rate is 0.2 mL·min^(-1),injection volume is 5μL,and column temperature is at 30℃.Mass spectrometry conditions are as follows:under negative ion mode,capillary voltage is 2.6 kV,drying gas temperature is at 180℃,drying gas flow rate is 6 L·min^(-1),auxiliary gas pressure is 0.08 MPa,mass-to-charge ratio collection ranges from 100 m·z^(-1)to 1,200 m·z^(-1),and collision energy is 35 eV.After analyzing 5 kinds of sorghum,20 kinds of sorghum polyphenols are identified,including ten kinds of phenolic acids,three kinds of proanthocyanidins,and seven kinds of phenolic acid derivatives.