近红外荧光碳点的合成及其在生物成像中的应用

Near-infrared emission carbon dots for bio-imaging applications

由于红光/近红外发射具有深层组织穿透力强、自体荧光小、对生物组织损伤小等特点,具有上述特性的碳点的制备与生物成像应用备受关注。本文以磺化四苯基卟啉为前驱体,采用溶剂热法合成近红外发射的荧光碳点(NIR-CDs)。NIR-CDs的最大发射峰位于692 nm,其荧光发射具有激发波长非依赖性,经分析NIR-CDs的近红外荧光发射主要源于分子态发光。此外,NIR-CDs还具有良好的水溶性和生物相容性、丰富的表面官能团、低毒性和优异的细胞标记能力,证实了NIR-CDs在细胞近红外成像中的应用潜力。本研究有望促进面向生物应用的近红外荧光碳点的发展,推动新型碳点的研究与实际应用。

It is very difficult to prepare red/near-infrared emission carbon dots(CDs)for bio-imaging applications which are needed because of their deep tissue penetration,minimal auto-fluorescence,and low emission light damage to bio-tissues.Near-infrared emitting CDs(NIR-CDs)were synthesized from sulfonated tetraphenylporphyrin using a solvothermal method.They have excitation-independent properties with a maximum emission at 692 nm.Studies showed that this unique near-infrared emission mainly originated from the aggregated molecular states of the CDs.The NIR-CDs showed good water solubility,exceptional biocompatibility,low toxicity,and superior cellular labelling ability.This work could significantly advance the structural design and preparation of NIR-CDs and corresponding bio-imaging applications.