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红参多糖分级醇沉组分对肺腺癌A549细胞增殖抑制作用 被引量:1

Effect of red ginseng polysaccharides in ethanol fractional precipitation on proliferation activity of A549 cells
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摘要 探究红参中分级醇沉多糖的体外抗肿瘤活性。采用水提醇沉法得红参粗多糖,用不同体积分数乙醇溶液(55%,60%,65%),分级沉淀得不同级分的红参多糖(RGP-55,RGP-60和RGP-65)。苯酚-硫酸法测定多糖含量,通过紫外、红外扫描进行纯度和结构分析。CCK-8法研究红参多糖对肺腺癌A549细胞的增殖抑制作用。结果:红参多糖RGP-55,RGP-60和RGP-65的多糖含量分别为70.29%,63.15%和56.23%。紫外扫描结果显示3种红参多糖均不含核酸和蛋白质。红外光谱分析结果表明,3种红参多糖结构中均含有多糖类物质的特征吸收峰。3种红参多糖均可抑制肺腺癌A549细胞的增殖,且呈时间和浓度依赖性。结论:红参中分离出的3种分级醇沉多糖对肺腺癌A549细胞具有增殖抑制作用。 To investigate antitumor activity in vitro of red ginseng polysaccharides in ethanol fractional precipitation.The polysaccharides were extracted with water,and precipitated with ethanol from red ginseng.RGP-55,RGP-60 and RGP-65 were precipitate by 55%,60%and 65%ethanol.The content of polysaccharides was measured by phenol-sulfuric acid method.The purity and structure of polysaccharides were analyzed by UV and IR spectroscopy.Their inhibitory effect on the growth of A549 cells was evaluated by CCK-8.Results:the polysaccharide contents of RGP-55,RGP-60 and RGP-65 were 70.29%,63.15%and 56.23%,respectively.Results of the UV spectroscopy show that none of the three polysaccharides contains impurities like nucleic acid and protein.Results of the IR spectroscopy show that three polysaccharides contains characteristic absorption peak of polysaccharides.Three polysaccharides can inhibit the growth of A549 cells in concentration and time dependent manners.Conclusion:Three different polysaccharides with the ability to inhibit A549 cells proliferation have been obtained from red ginseng.
作者 崔海洋 张兴超 张雪茹 翟凤国 刘嘉祺 刘佳维 CUI Haiyang;ZHANG Xingchao;ZHANG Xueru;ZHAI Fengguo;LIU Jiaqi;LIU Jiawei(School of Pharmaceutical,Mudanjiang Medical University,Mudanjiang 157011,China)
出处 《实验室科学》 2021年第2期55-58,64,共5页 Laboratory Science
基金 黑龙江省省属高等学校基本科研业务费科研项目(项目编号:2020-KYYWF-0802) 黑龙江省大学生创新创业训练计划项目(项目编号:202010229042) 牡丹江市应用技术研究与开发计划项目(项目编号:SQ2020NS085)。
关键词 红参多糖 分级醇沉 多糖含量 抗肿瘤活性 red ginseng polysaccharide ethanol fractional precipitation polysaccharide content antitumor activity
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