胞核中核转运受体蛋白Karyo-pherin alpha 2水平与胃癌预后的关系研究

Effect of Karyopherin alpha 2 expression in nucleus on prognosis of gastric cancer

目的探讨核转运受体蛋白Karyopherin alpha 2(KPNA2)水平与胃癌患者各临床病理参数及预后的关系。方法选取2010年1月至2014年11月浙江省台州医院行手术切除的胃癌患者肿瘤石蜡包埋标本103例,制成组织芯片,使用免疫组化法检测胃癌及癌旁组织与正常组织中KPNA2与p53的表达情况。分析KPNA2表达与胃癌患者各项临床病理参数及预后的关系,分析KPNA2与p53表达的关系。结果胃癌组织细胞核中KPNA2阳性率高于癌旁组织、正常组织。KPNA2表达与患者年龄、肿瘤大小、神经侵犯、脉管内癌栓、肿瘤的浸润深度、淋巴结有无远处转移、TNM分期等均相关(均P<0.05),与患者性别、组织学分级、组织学分类、淋巴结转移、切缘情况、Lauren分型等均无关(均P>0.05)。KPNA2核表达阳性组患者的生存时间低于阴性组(P<0.05)。此外KPNA2核表达与p53在蛋白水平和mRNA水平上相关(均P<0.05)。结论KPNA2核表达与胃癌临床病理参数及预后较差相关,与p53表达相关,提示KPNA2可能是胃癌患者的其中一个预后分子标志物以及潜在治疗靶点。

Objective To explore the association of expression of nuclear transporter receptor protein Karyopherin alpha 2(KPNA2)with clinicopathological features and prognosis of gastric cancer.Methods One hundred and three patients with gastric cancer who underwent complete resection in Taizhou Hospital of Zhejiang Province from January 2010 to November 2014 were enrolled in the study.The formalin-fixed,paraffin-embedded(FFPE)tissue samples were used for tissue microarray examination.The expression of KPNA2 and p53 in gastric cancer,adjacent tissues and normal tissues were detected by immunohistochemistry.The relationship of KPNA2 expression with clinicopathological features and prognosis of patients was analyzed.Results The positive rate of KPNA2 expression in the nuclei of gastric cancer tissues was higher(49/103,48.6%)than that in adjacent tissues and normal tissues.The expression of KPNA2 was significantly correlated with age,tumor size,nerve invasion,tumor thrombus,tumor invasion depth,and distant lymph node metastasis and TNM stage(all P<0.05),but was not correlated with gender,histological grade,histological classification,lymph node metastasis,incision margin and Lauren classification(all P>0.05).The survival time of KPNA2 positive group was shorter than that of negative group(P<0.05).In addition,the expression of KPNA2 was also correlated with that of p53(P<0.05).Conclusion The nuclear expression of KPNA2 is correlated with clinicopathological parameters and poor prognosis of gastric cancer,and is correlated with the expression of p53.It suggests that KPNA2 may be one of the prognostic molecular markers and a potential therapeutic target for gastric cancer.