吉林地区奶牛子宫内膜炎致病菌的分离鉴定及敏感药物筛选

Isolation and Identification of Pathogenic Bacteria of Dairy Cow Endometritis in Jilin Area and Screening of Sensitive Drugs

为科学有效防控奶牛子宫内膜炎,本课题组采集了吉林地区多个奶牛养殖场120头患子宫内膜炎奶牛子宫内容物样本,并对其进行染色、镜检以及生化鉴定。分离出主要致病菌为大肠杆菌、金黄色葡萄球菌、表皮葡萄球菌、链球菌以及蜡样芽胞杆菌,单一感染、双重感染所占比例分别为44.35%和41.74%,三重感染所占比例为13.91%;通过敏感药物筛选头孢噻肟、庆大霉素、环丙沙星、四环素、阿莫西林、恩诺沙星以及氧氟沙星均显示出较好的抑菌效果。以上药物可作为吉林地区奶牛子宫内膜炎治疗的首选药物,科学有效防控该病有利于奶牛养殖业和谐、快速、健康发展。

To prevent and control endometritis in dairy cows,samples of uterine contents from 120 cows suffering endometritis in several dairy farms in Jilin area were collected for microscopically examination and biochemically identification.The main pathogenic bacteria isolated were Escherichia coli,Staphylococcus aureus,Staphylococcus epidermidis,Streptococcus and Bacillus cereus.The proportion of single infection and double infections was 44.35%and 41.74%respectively,and the proportion of triple infection was 13.91%.Through screening sensitive drugs,we found that cefotaxime,gentamicin,ciprofloxacin,tetracycline,amoxicillin,enrofloxacin and ofloxacin all showed good antibacterial effects,which can be used as the first choice of drugs for the treatment of dairy cow endometritis in Jilin area,scientific and effective prevention and control of this disease is conducive to the harmonious,rapid and healthy development of dairy farming.