不同剂量地西他滨对骨髓增生异常综合征小鼠Beclin1蛋白表达的影响

Effects of different doses of decitabine on Beclin1 protein expression in mice with myelodysplastic syndrome

目的:探讨不同剂量地西他滨对骨髓增生异常综合征(MDS)小鼠Beclin1蛋白表达的影响。方法:选择36只NOD/SCID小鼠制备MDS小鼠模型后,随机分为模型组、低剂量组和高剂量组,每组12只。模型组、低剂量组、高剂量组隔天于腹腔内分别注射5 mg/kg生理盐水、5 mg/kg地西他滨、10 mg/kg地西他滨,治疗28 d。比较三组小鼠体重、红细胞(RBC)、白细胞(WBC)、血小板(PLT)以及Beclin1蛋白表达情况。结果:治疗后,低剂量组和高剂量组小鼠第14、28天体重、RBC、WBC、PLT高于模型组(均P<0.05),CD 45+细胞比例低于模型组(P<0.05),第28天小鼠肝、脾组织Beclin1蛋白相对表达水平高于模型组(均P<0.05),而低剂量组与高剂量组上述指标比较差异无统计学意义(均P>0.05)。结论:低剂量地西他滨即可提高MDS小鼠Beclin1蛋白表达水平,抑制增殖与浸润,从而促进小鼠外周血与体重恢复正常。

Objective:To investigate the effects of different doses of decitabine on the expression of Beclin1 in mice with myelodysplastic syndrome(MDS).Methods:After selecting 36 NOD/SCID mice to prepare MDS mouse models,they were randomly divided into model group,low-dose group and high-dose group,with 12 mice in each group.The model group,the low-dose group,and the high-dose group were injected intraperitoneally with 5 mg/kg saline,5 mg/kg decitabine,and 10 mg/kg decitabine respectively every other day for 28 days of treatment.The weight,red blood cell(RBC),white blood cell(WBC),platelet(PLT)and Beclin1 expression of the three groups were compared.Results:After treatment,the body weight,RBC,WBC,and PLT of mice in the low-dose group and high-dose group were higher than those in the model group on the 14th and 28th days(all P<0.05),and the ratio of CD 45+cells was lower than that in the model group(P<0.05),and the relative expression levels of Beclin1 in the liver and spleen tissues of mice on the 28th day were higher than those in the model group(all P<0.05),but there was no significant difference in the above indicators between the low-dose group and the high-dose group(all P>0.05).Conclusion:The application of low-dose decitabine can increase the expression level of Beclin1 in mice with MDS,inhibit proliferation and infiltration,and promote the recovery of peripheral blood and body weight in mice.