相思藤醇提物对四氯化碳诱导肝纤维化大鼠的作用研究

Effectofethanol extract of Xiangsiteng on carbon tetrachloride-induced liver fibrosis in rats

目的:研究相思藤醇提物对四氯化碳(CCl4)诱导的肝纤维化大鼠的治疗作用。方法:取SD大鼠,灌胃给予50%CCl4花生油溶液(1 mL/kg)复制肝纤维化模型。将肝纤维化大鼠随机分为模型组、阳性组(秋水仙碱)及相思藤醇提物高、中、低剂量组,每组10只,另设正常组大鼠10只。各给药组灌胃给予相应药物治疗40 d。正常组、模型组大鼠灌胃给予等体积的生理盐水。末次给药24 h后,检测各组血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)、白球比(A/G)、透明质酸(HA)、层黏连蛋白(LN)、Ⅲ型胶原(colⅢ)、Ⅳ型胶原(colⅣ)的含量。苏木精-伊红(HE)染色和Masson染色观察肝组织病理学变化。结果:与正常组比较,模型组大鼠肝脏指数、ALT、AST、ALP、HA、LN、colⅢ、colⅣ显著上升,A/G明显下降(P<0.05或P<0.01)。与模型组比较,给药组大鼠肝脏指数、ALT、AST、ALP、HA、LN、colⅢ、colⅣ明显下降,A/G明显上升(P<0.05或P<0.01),肝组织病变及纤维增生减少。结论:相思藤醇提物能改善肝纤维化大鼠的肝功能,减少肝脏组织病理学改变及纤维增生。

Objective:To research the effect of ethanol extract of Xiangsiteng on carbon tetrachloride(CCl4)-induced liver fibrosis in rats.Methods:SD rats were given 50%CCl4 peanut oil solution(1 mL/kg)by gavage to establish liver fibrosis model.Rats with liver fibrosiswere randomly divided into a model group,a positive control drug(colchicine)group,and ethanol extract of Xiangsiteng high,medium,and low dose groups,10 rats per group.A normal group(10 rats)was also set.Each rat in the drug groups was given the corresponding drug by gavage for 40 consecutive days.Rats in the normal group and model group were given equal volume of normal saline.24 hours after the last administration,the aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP),hyaluronic acid(HA),laminin(LN),collagenⅢ(colⅢ)and collagenⅣ(colⅣ)levels in serum as well as the white ball ratio(A/G)were detected.The pathological changes of liver tissues were observed by hematoxylin-eosin(HE)staining and Masson staining.Results:Compared with the normal group,the liver index,ALT,AST,ALP,HA,LN,colⅢ,colⅣof the rats in model group increased significantly,but the A/G decreasedsignificantly(P<0.05 or P<0.01).Compared with the model group,the liver index,ALT,AST,ALP,HA,LN,colⅢ,colⅣof the rats in the administration groups decreased significantly,but the A/G increased significantly(P<0.05 or P<0.01).The liver tissue lesions and fibrous hyperplasia were reduced as well.Conclusion:Ethanol extract of Xiangsiteng can improve the liver function,reduce the histopathological changes and fibrous hyperplasia of liver in rats with liver fibrosis.