miR-30e-3p在弥漫大B细胞淋巴瘤microRNA芯片集测序数据中的表达及机制研究

Expression and biological mechanism of miR-30e-3p in the Chip-seq data of diffuse large Bcell lymphoma

目的:探究miR-30e-3p在弥漫大B细胞淋巴瘤(DLBCL)的表达意义和潜在分子机制。方法:收集全球范围内4个DLBCL miRNA芯片和miRNA-seq数据集数据,通过计算整合的标准化均数差(SMD)和绘制汇综合受试者工作特征曲线(SROC曲线),检测miR-30e-3p的表达。将miRWalk预测的miR-30e-3p潜在靶标和DLBCL的上调差异基因(DEGs)的交集基因进行功能富集分析。通过6个mRNA基因芯片和RNA-seq数据集验证靶基因的mRNA水平及其与miR-30e-3p的关系。结果:miR-30e-3p在DLBCL组(108个样本)的表达明显低于非肿瘤组(35个样本)(SMD=-2.33)。低表达miR-30e-3p有显著的区分DLBCL和非肿瘤的能力(AUC=0.96)。103个miR-30e-3p潜在靶基因中CDC25A为核心基因,其在171例DLBCL的表达在mRNA水平明显上升(SMD=0.72,AUC=0.93),与miR-30e-3p表达呈明显负相关关系(r=-0.3204,P=0.0281)。结论:miR-30e-3p可能通过负向调控其潜在靶基因CDC25A参与DLBCL的发生发展。

Objective:To explore the expression significance and potential molecular mechanism of miR-30e-3p in diffuse large B-cell lymphoma(DLBCL).Methods:The expression of miR-30e-3p were assessed by Standardized Mean Difference(SMD)and drawing a summarized receiver operating characteristic(SROC)curve,using data available from 4 miRNAarray and miRNA-sequencing datasets.Intersection of the miR-30e-3p potential target genes forecasted from miRWalk,and differentially upregulated genes in DLBCL were used to functional enrichment analysis.Six miRNA array and miRNA-sequencing datasets were applied to verify themRNA level of the tagert gene and the relationship with miR-30e-3p.Results:The expression of miR-30e-3p in the DLBCL group(108 samples)was significantly lower than that in the noncancer group(35 samples)(SMD=-2.33),and the downregulation of miR-30e-3p showed excellent ability in difffferentiating between the two groups(AUC=0.96).CDC25A was the core gene among 103 miR-30e-3p potential target genes.Expression of CDC25A in the 171 DLBCL samples was significantly high(SMD=0.72,AUC=0.93)and a statistically signifificant negative correlation was found between miR-30e-3p and CDC25A(r=0.3204,P=0.0281).Conclusion:miR-30e-3p may participate in the occurrence and development of DLBCL through negative regulation of its potential target gene CDC25A.