成纤维细胞激活蛋白在肾间质纤维化大鼠肾脏组织中的表达及意义

The expression and significance of fibroblast activating protein in renal tissue of rats with re⁃nal interstitial fibrosis

目的:探讨成纤维细胞激活蛋白(FAP)在肾间质纤维化(RIF)大鼠肾脏组织中的表达及意义。方法:将20只雄性SD大鼠随机分为假手术组和模型组,每组10只。模型组结扎左侧输尿管,假手术组仅开腹不结扎。2周后处死大鼠,取左肾组织进行病理学检查并计算RIF指数,分别采用实时荧光定量PCR法(qPCR)和Western blotting法检测大鼠肾组织中FAP、α-平滑肌肌动蛋白(α-SMA)、转化生长因子-β1(TGF-β1)mRNA和蛋白表达。结果:与假手术组比较,模型组肾组织重度萎缩,大量结缔组织增生,伴有大量炎性细胞浸润,RIF指数显著升高,肾组织FAP、α-SMA和TGF-β1 mRNA和蛋白表达均明显升高(均P<0.05)。Pearson相关分析显示,模型组肾组织FAP、α-SMA和TGF-β1蛋白表达与RIF指数均呈显著正相关关系(均P<0.05),FAP蛋白表达与α-SMA和TGF-β1蛋白表达亦呈显著正相关关系(均P<0.05)。结论:FAP在RIF大鼠肾脏组织中的表达显著升高,且可能参与了RIF的发生和发展。

Objective:To investigate the expression and significance of fibroblast activating protein(FAP)in renal tissue of rats with renal interstitial fibrosis(RIF).Methods:Twenty male SD rats were randomly divided into sham operation group and model group,with ten rats in each group.Rats in model group were ligated with left ureter;and rats in sham operation group were only opened the abdominal cavity without ligation.All rats were sacrificed after 2 weeks.The left renal tissue was taken for pathological examination and RIF index calculating.The expressions of FAP,α-smooth muscle actin(α-SMA)and transforming growth factor–beta 1(TGF-β1)in renal tissue were detected by using real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)and western blotting.Results:Compared with the sham operation group,the kidney tissue in the model group was severe atrophy,massiveconnective tissue proliferated with numerousinflammatory cell infiltration,RIF index increased significantly.The mRNA and proteinexpressions of FAP,α-SMA and TGF-β1 in kidney tissue were significantly increased(P<0.05).Pearson correlation analysis showed that the expressions of FAP,α-SMA and TGF-β1 protein in renal tissues of the model group were significantly positively correlated with the RIF index(P<0.05),FAP protein expression was also significantly positively correlated withα-SMA and TGF-β1 protein expression(P<0.05).Conclusion:The expression of FAP in the kidney tissue of RIF rats is significantly increased,which may be involved in the occurrence and development of RIF.