白藜芦醇抑制肾移植大鼠肾损伤

Resveratrol inhibits kidney injury in renal transplantation rats

目的探究白藜芦醇对肾移植大鼠肾损伤的改善作用及作用机制。方法将40只大鼠分为假手术组、模型组(同种异体肾移植手术)、白藜芦醇高和低剂量组[40和20 mg/(kg·d),腹腔注射,连续7 d]。术后24 h,检测血清Cr、BUN、SOD和MDA水平,RT-qPCR检测肾组织Bax和Bcl-2 mRNA表达,Western blot检测IKKα、p-IκBα和NF-κB p65蛋白表达。结果与假手术组比较,模型组血清Cr、BUN和MDA含量增高,SOD活性降低,肾组织Bax mRNA表达增加,Bcl-2 mRNA表达降低,IKKα、p-IκBα和NF-κB p65蛋白表达增加(P<0.05);与模型组比较,白藜芦醇显著降低血清Cr、BUN和MDA含量,提高SOD活性,降低Bax mRNA、IKKα、p-IκBα和NF-κB p65蛋白表达,提高Bcl-2 mRNA表达(P<0.05)。结论白藜芦醇对肾移植大鼠肾损伤具有保护作用,其作用机制与抑制NF-κB信号通路的活化有关。

Objective To explore the effect and mechanism of resveratrol on improving kidney injury in renal transplantation rats.Methods Forty rats were divided into sham group,model group(allogeneic kidney transplantation),high and low dose resveratrol groups[40 and 20 mg/(kg·d),intraperitoneal injection,for 7 consecutive days].Twenty four hours after operation,the serum level of Cr,BUN,SOD and MDA was detected,the expression of Bax and Bcl-2 mRNA in renal tissues was tested by RT-qPCR,and the protein expression of IKKα,p-IκBα,and NF-κB p65 was detected by Western blot.Results Compared to the sham group,serum Cr,BUN and MDA level in the model group increased,SOD activity decreased,Bax mRNA expression in renal tissue increased,Bcl-2 mRNA expression decreased,and the expression of IKKα,p-IκBα,NF-κB p65 protein increased(P<0.05);compared with the model group,resveratrol significantly reduced serum Cr,BUN and MDA content,while increased SOD activity,reduced Bax mRNA and IKKα,p-IκBα,NF-κB p65 protein expression,and increased Bcl-2 mRNA expression(P<0.05).Conclusions Resveratrol has a protective effect on kidney injury in kidney transplantation rats,and the underlying mechanism is potentially related to the inhibition of activation of NF-κB signaling pathway.