基于miRNA芯片对系统性红斑狼疮的生物信息学分析

Bioinformatic analysis of systemic lupus erythematosus based on miRNA microarray

目的运用生物信息学分析系统性红斑狼疮(SLE)患者单核细胞起源的树突状细胞(moDCs)中miRNA与健康人的表达差异。方法从公共基因芯片数据库(GEO)中下载GSE79240数据集,R语言筛选差异miRNA,Funrich软件分析差异miRNA的GO富集、KEGG信号通路及转录因子,MiRTarBase数据库进行靶基因预测。结果筛选出19个差异miRNAs,其中11个上调,8个下调。GO富集显示差异miRNA的生物学功能主要集中在信号传导及细胞间通讯;细胞组成主要位于细胞核及细胞质;分子功能主要集中在转录因子、泛素特异性蛋白酶及受体信号复合物支架。KEGG信号通路主要集中在蛋白多糖信号通路、TRAIL信号通路及S1P信号通路。与miRNA有关联的转录因子主要包括:SP1、SP4、EGR1及POU2F1。14个miRNA经实验验证有靶基因。结论在SLE患者的moDCs中发现19个差异miRNAs,可能在SLE的发病机制中起到了重要作用,为SLE的诊断提供新的思路。

Objective To analyze the differential expressions of miRNA in monocyte derived dendritic cells(moDCs)from systemic lupus erythematosus(SLE)patients and healthy people by bioinformatic analysis.Methods The GSE79240 dataset was downloaded from the gene expression omnibus(GEO)database,differential miRNAs were screened by R language.Funrich software was used to analyze the gene ontology(GO)enrichment,Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment and transcription factors.MiRTarBase database was used to predict target genes.Results A total of 19 differential miRNAs were screened out,of which 11 were up-regulated and 8 were down-regulated.Biological process of GO enrichment was enriched in signal transduction and cell communication;cellular component of GO enrichment was enriched in nucleus and cytoplasm;molecular function of GO enrichment was expressed in transcription factor activity,ubiquitin-specific protease activity and receptor signalling complex scaffold activity.KEGG pathway was shown mainly in glypican signalling pathway,TRAIL signalling pathway and S1P signalling pathway.The transcription factors related to miRNA mainly included:SP1,SP4,EGR1 and POU2F1.Forteen miRNAs have been experimentally verified to have target genes.Conclusions The differential miRNAs in moDCs of SLE patients may play an important role in the pathogenesis of SLE and provide new clue for the diagnosis of SLE.