根系分泌物对紫云英油菜间作的响应

Responses of root exudates to intercropping of Chinese milk vetch with rape

根系分泌物是植物与土壤进行物质交换和信息传递的重要载体,也是间作体系中作物-土壤-微生物互作的重要调控者。为进一步揭示间作体系中作物之间的互作机制,本研究通过紫云英单作、油菜单作和紫云英油菜间作,重点分析了紫云英油菜间作下根系分泌物的响应特征。结果表明:共检测到紫云英和油菜根系分泌物391种,定性93种,包括了9种代谢物类型,其中有机氧化物含量最高,主要是以核糖醇的形式存在。不同种植模式中,紫云英、油菜的根系分泌物含量差异显著,紫云英油菜间作时根系分泌物特征与油菜单作相似,与紫云英单作差异较大。不同种植模式的差异根系分泌物中,仅9-芴酮1与其他差异分泌物间呈负相关关系。不同种植模式的差异根系分泌物主要为苯系物、脂类和类脂分子、有机酸及其衍生物、有机氧化物等,其中苯系物、脂类和类脂分子是表征紫云英、油菜根系分泌物变化的重要类型。可见,紫云英油菜间作改变了作物的根系分泌物特征,其变化特征与苯系物、脂类和类脂分子关系密切。

Root exudates are important carriers for material exchange and information transfer between plant and soil, and important regulators of crop-soil-microorganism interaction in intercropping systems. We examined the interaction between crops in intercropping system by setting three treatments, monoculture Chinese milk vetch, monoculture rape and Chinese milk vetch intercropped with rape. The responses of root exudates were emphatically analyzed. The results showed that 391 root exudates were detected, with 93 of which being identified and divided into nine types of metabo-lites. Among them, organooxygen compounds were the most abundant, mainly in the form of ribitol. Under different planting patterns, root exudates of Chinese milk vetch and rape were significantly different. The characteristics of root exudates in intercropping were similar to monoculture rape, but significantly different from monoculture Chinese milk vetch. Among the root exudates in different planting modes, only 9-fluorenone 1 was negatively correlated with others. The differential root exudates were mainly benzenoids, lipids and lipid-like molecules, organic acids and derivatives, and organooxygen compounds. The benzenoids, lipids and lipid-like molecules were important types that characterized the changes of root exudates of Chinese milk vetch and rape. Chinese milk vetch intercropping with rape changed the characteristics of root exudates, which were closely related to benzenoids, lipids, and lipid-like molecules.