USP 12促进循环牵张力作用下人牙周膜干细胞的成骨分化过程

USP 12 Promotes Osteogenic Differentiation of Human Periodontal Ligament Stem Cells under Cyclic Tension

目的:探究泛素特异性蛋白酶12(USP 12)在循环牵张力刺激引起的人牙周膜干细胞(hPDLSCs)成骨分化中的作用。方法:原代培养健康的hPDLSCs,通过成骨、成脂及成软骨诱导分化实验检测其干细胞分化特性。使用循环牵张力刺激hPDLSCs 24 h、48 h、72 h,实时定量PCR、免疫印迹和免疫荧光实验检测USP 12的表达变化,ALP染色检测hPDLSCs的成骨分化,实时定量PCR检测成骨相关因子ALP、OGT、OPN的表达变化。结果:诱导分化试验染色显示本研究使用的原代hPDLSCs具有多向分化潜力。循环牵张力刺激hPDLSCs后,USP 12的表达均呈先升高后降低的趋势。在循环牵张力刺激下hPDLSCs发生成骨分化,ALP、OGT的表达先升高后降低,OPN逐渐升高。结论:USP 12可能促进循环牵张力作用下人牙周膜干细胞的成骨分化。

Objective:To investigate the influence of ubiquitin-specific protease 12(USP 12)on osteogenic differentiation of human periodontal ligament stem cells(hPDLSCs)under cyclic tension.Methods:Primary hPDLSCs were collected and the differentiation characteristics were detected by osteogenic,adipogenic,and chondrogenic differentiation experiments.The expression of USP 12 was detected by real-time quantitative PCR,Western blot,and immunofluorescence assay.The osteogenic differentiation of hPDLSCs was detected by ALP staining test.The expressions of ALP,OGT,and OPN were detected by real-time quantitative PCR.Results:The primary hPDLSCs had the potential of multi-directional differentiation.The expression of USP 12 increased at first and then decreased after cyclic stretch stimulation.The expression of ALP and OGT increased at first and then decreased,and the expression of OPN increased gradually.Conclusion:USP 12 may promote osteogenic differentiation of hPDLSCs under cyclic tension.