运用制动法建兔KOA分期模型的合理性分析

Analysis of the rabbit knee osteoarthritis staging model using the braking method

目的石膏制动建立膝骨关节炎模型的不同分期,以求为针灸和中医药的精准治疗提供适当的动物模型。方法选用40只大耳白兔,随机分为模型1、2、3、4组,每组样本量均为10只。均采取左后肢伸直位制动,制动分别为10 d、20 d、30 d、40 d,观察兔膝关节的行为、形态和病理表现。结果模型各组间的Lequesne MG行为学评分、Jessica Badendick大体形态评分变化显著(P<0.05),病理检测结果各组阶段性、差异性明显。结论在膝关节骨性关节炎的造模过程中,各组间差别显著,根据其各阶段的最显著表现,可大致把疾病分为初期、早期、中期、晚期,在准确辨期的基础上加以针灸或中医药的干预可能有效治疗该病。

Objective We used plaster immobilization to establish different stages of the knee osteoarthritis model to provide an appropriate animal model for precise treatment of acupuncture and traditional Chinese medicine.Methods A total of 40 large-eared white rabbits were selected and randomly divided into model groups(1,2,3,and 4),with a sample size of 10 in each group.The left hind limbs were immobilized in the extended position.Immobilization lasted for 10 days,20 days,30 days,and 40 days,respectively.The behavior,morphology,and pathological manifestations of the rabbits’knee joints were observed.Results Lequesne myasthenia gravis behavior scores and Jessica Badendick general morphology scores changed significantly in each model group(P<0.05).The pathological test result showed a significant phase and difference among the groups.Conclusions In the process of modeling knee osteoarthritis,there are significant differences between the groups.According to the most significant manifestations of each stage,the disease can be roughly divided into an early stage,middle stage,and late stage.Intervention of acupuncture or traditional Chinese medicine may be effective in treating the disease.