CKIP-1对小鼠颅颌面软硬组织生长发育的影响

The effects of casein kinase 2 interacting protein-1 on the growth and development of craniomaxillofacial soft and hard tissues in mice

目的探讨酪蛋白激酶2相互作用蛋白1(the casein kinase 2 interacting protein-1,CKIP-1)基因对颅颌面部软硬组织的影响,为颅颌面相关疾病的机制研究和治疗提供基础。方法 6月龄雄性CKIP-1基因敲除小鼠(KO)作为实验组,野生型小鼠(WT)作为对照组,用Micro-CT对小鼠颅颌面部硬组织(顶骨、鼻骨、切牙、磨牙)的骨量进行分析,另外用HE染色和甲苯胺蓝染色分析颌面部软组织(鼻软骨、唇黏膜、舌)的组织形态差异。结果 CKIP-1基因可负调控小鼠颅颌面骨松质骨的骨量及牙齿的牙本质矿化,相较WT小鼠,KO小鼠的顶骨板障层厚度增加93%,而皮质骨未见明显差异;鼻骨松质骨厚度增加160%,皮质骨无显著差异;牙釉质厚度未见异常,但牙髓腔变小,牙本质厚度增加48%;HE及甲苯胺蓝染色分析发现KO小鼠的鼻翼软骨板厚度增加57%,并发现局部出现异常骨化;唇黏膜角化层增厚170%;舌肌肌纤维直径增加45%。结论 CKIP-1基因对小鼠颌面部多种软硬组织的生长发育均有不同程度的影响。

Objective To investigate the effect of casein kinase 2 interacting protein-1(CKIP-1)on craniofacial soft tissues and hard tissues,to provide the basis for the study and treatment of craniomaxillofacial related diseases.Methods 6-month-old male CKIP-1 knockout(KO)mice were selected as the experimental group,and wild-type(WT)mice were selected as the control group.The craniomaxillofacial hard tissues(parietal bone,nasal bone,incisors and molars)were analyzed through micro-CT,and the morphological changes of maxillofacial soft tissues(nasal cartilage,lip mucosa and tongue)were analyzed through HE staining and toluidine blue staining.ResultsCKIP-1 negatively regulated bone mass of cancellous bone of cranial and maxillofacial bones and dentin mineralization.Compared with the WT mice,the thickness of the parietal baffle layer increased by 93%in KO mice,while cortical bone showed no significant difference between the two groups.The nasal cancellous bone thickness increased by 160%in KO-mice,while cortical bone showed no significant difference between the two groups;the enamel thickness was normal,but the pulp cavity became smaller and the dentin thickness increased by 48%.Compared with the WT mice,the HE staining and toluidine blue staining analyses of the soft tissues revealed that the thickness of the alar cartilage plate of KO mice increased by 57%,and local ossification was found within the cartilage plate.The thickness of the keratinized layer of the labial mucosa increased by 170%in KO mice and the muscle fiber diameter of the lingual muscle increased by 45%.Conclusion CKIP-1 genes have different effects on the growth and development of various soft and hard tissues in the maxillofacial region of mice.