不同功能状态的桥本氏甲状腺炎患者甲状腺球蛋白抗体糖基化修饰的差异研究

Glycosylation of thyroglobulin antibody in patients with different functional Hashimoto's thyroiditis

目的探讨甲状腺功能正常,亚临床甲状腺功能减退和甲状腺功能减退三种不同状态的桥本氏甲状腺炎(Hashimoto's thyroiditis,HT)患者血清中甲状腺球蛋白抗体(thyroglobulin antibody,TgAb)免疫球蛋白(immunoglobulin,Ig)G糖基化修饰水平的差异。方法收集天津市第五中心医院2016年12月至2019年8月诊断的TgAb阳性的初发HT患者96例,根据甲状腺功能不同将患者分为甲状腺功能正常组(Eu组,n=32),亚临床甲状腺功能减退组(sH组,n=28)和甲状腺功能减退组(H组,n=36),采用抗原特异的凝集素酶联免疫吸附测定法(lectin-enzyme-linked immunosorbent assay,L-ELISA)分别检测TgAb上的岩藻糖、半乳糖及唾液酸的相对含量。结果Eu组患者TgAb IgG的阳性率低于H组(35.7%比52.7%,P=0.016),但在sH组与H组中差异无统计学意义(46.8%比52.7%,P=0.396)。Eu组,sH组和H组的TgAb末端的核心岩藻糖含量的中位数分别是18.3%,17.7%和16.8%,半乳糖含量的中位数分别是40.2%,38.4%和39.6%,唾液酸含量的中位数分别是98.4%,84.3%和89.6%,其差异均无统计学意义(P值均>0.05)。结论不同功能状态的HT患者TgAb IgG的糖基化修饰水平虽没有显著差异,但提示了对TgAb IgG Fc段糖基化修饰的精确研究可能是下一步探讨不同功能状态HT发生机制的方法。

Objective To investigate the glycosylation of sera Thyroglobulin antibody(TgAb)in Hashimoto's thyroiditis(HT)patients with normal thyroid function,subclinical hypothyroidism and hypothyroidism.Methods A total of 96 newly diagnosed HT patients diagnosed as TgAb in Tianjin Fifth Central Hospital from December 2016 to August 2019 were collected and divided into euthyroidism groups(Eu group,n=32),subclinical groups(sH group,n=28)and overt hypothyroidism groups(H group,n=36).Lectin-enzyme-linked immunosorbent assay(L-ELISAS)were performed to detect the relative amount of core fucose,terminal galactose,and sialic acid on each TgAb respectively.Results The prevalence of serum TgAb IgG in Eu groups was significantly lower than that in H groups(35.7%vs 52.7%,P=0.016).But there was no significant difference onTgAbIgG between sH and H groups(46.8%vs 52.7%P=0.396).Among Eu,Sh,H groups,the median contents of core fucose inTgAbterminal were 18.3%,17.7%and 16.8%;The median contents of terminal galactose were 40.2%,38.4%and 39.6%;The median contents of sialic acid were 98.4%,84.3%and 89.6%,respectively.There was no significant difference in the three carbohydrate residue contents on sera TgAb among Eu,sH,H groups(all P values>0.05).Conclusion There is no significant difference in glycosylation of seraTgAbamong different functional HT,while the precision study of glycosylation of the IgG-Fc maybe the next step in patients with different functional HT.