基于高通量测序分析昆明官渡区市售芫荽细菌污染状况

Investigation on the situation of bacterial contamination in coriander sold in Guandu district of Kunming city based on high-throughput sequencing

目的采用高通量测序技术分析昆明市官渡区市售芫荽细菌污染状况。方法以昆明市官渡区超市及农贸市场售芫荽为样本,采用高通量16S rDNA扩增子测序技术结合FAPROTAX功能群体预测分析市售芫荽样品细菌多样性,重点关注潜在致病菌以及人类肠道菌群。结果从全部样品共获得833个操作分类单元(operational taxonomic units, OTUs),经物种注释可归入细菌的19个门、288个属。其中在全部或部分芫荽样品中检测到以下潜在致病菌OTUs:致腹泻和肠胃炎细菌,如布氏弓形杆菌、脆弱拟杆菌、产碱普罗威登斯菌等;致医院感染条件致病菌,如抗辐射不动杆菌、土壤不动杆菌和奥斯陆莫拉氏菌。此外还检测到多种人类肠道菌群的OTUs,如产气柯林斯菌、长链多雷尔氏菌、大肠埃希氏菌等。结论简单焯水或清洗后食用芫荽的饮食习惯存在巨大的食品安全隐患,芫荽可能已成为食源性疾病传播的一条重要途径,但并未受到足够重视。

Objective To investigate the situation of bacterial contamination in coriander sold in Guandu district of Kunming city based on high-throughput sequencing by high-throughput sequencing technology. Methods Taking cilantro samples sold in supermarkets and farmers’ markets in Guandu district of Kunming city as samples, the bacterial diversity of commercial cilantro samples was analyzed by using high-throughput 16 S rDNA ampland sequencing technology combined with FAPROTAX functional population prediction, with a focus on potential pathogenic bacteria and human intestinal flora. Results A total of 833 operational taxonomic units(OTUs) were observed. A total of 19 phyla and 288 genera were identified from the OTUs. The results showed that the following potential pathogenic bacteria OTUs were detected in all or part of coriander samples: Diarrhea and gastroenteritis(e.g. Arcobacter butzleri, Bacteroides fragilis, Providencia alcalifaciens and so on), and nosocomial infection(e.g. Acinetobacter radioresistens, Aci. soli and Moraxella osloensis). Furthermore, various human intestinal bacteria OTUs were also detected, e.g. Collinsella aerofaciens, Dorea longicatena, Escherichia coli, and so on. Conclusion Eating coriander after simply blanching or washing may bring hidden troubles for food safety and may have become an important route of transmission of foodborne diseases, but it doesn’t seem to arouse sufficient attention yet.