高效液相色谱-二极管阵列检测器测定兽药、保健食品中茶碱、二羟丙茶碱和盐酸二氧丙嗪

Determination of theophylline, diprophylline and dioxopromethazine in veterinary drugs and health food by high performance liquid chromatography-photodiode array detector

目的建立高效液相色谱-二极管阵列检测器(high performance liquid chromatography-photodiode array detector,HPLC-PDA)检测茶碱、二羟丙茶碱和盐酸二氧丙嗪3种平喘类药物成分的检测方法。方法以水-甲醇(70:30,V:V)为流动相,流速为1.0 m L/min,检测波长为272 nm同时测定茶碱和二羟丙茶碱;以0.025 mol/L磷酸溶液-乙腈(72:28,V:V)为流动相,流速为1.0 m L/min,检测波长264 nm测定盐酸二氧丙嗪。结果茶碱和二羟丙茶碱能得到良好分离,在1~50μg/mL范围内线性关系良好,相关系数大于0.9999,在不同样品中的回收率分别为95.9%~105.9%、95.4%~104.2%。盐酸二氧丙嗪在1~50μg/m L范围内线性关系良好,相关系数大于0.9990,在不同样品中的回收率在95.9%~104.8%之间。结论本方法操作简单,准确度高,可对兽药、保健食品中非法添加的3种平喘类药物进行定性和定量检测。

Objective To establish a method for the determination of 3 kinds of antiasthmatic drug components(theophylline, diprophylline and dioxopromethazine) by high performance liquid chromatography-photodiode array detector(HPLC-PDA). Methods Theophylline and diprophylline were simultaneously determined on conditions as follows: water-methanol(70:30, V:V) was used as the mobile phase, the flow rate was 1.0 mL/min, and the detection wavelength was 272 nm. Dioxopromethazine was determined on conditions as follows: 0.025 mol/L phosphoric acid acetonitrile(72:28, V:V) was used as the mobile phase, the flow rate was 1.0 mL/min, and the detection wavelength was 264 nm. Results Theophylline and diprophylline could be separated well. The linear range was 1-50 μg/mL, and the correlation coefficient was more than 0.9999. The recoveries of theophylline and diprophylline in different samples were 99.25.9%-101.85.9% and 96.05.4%-103.64.2%, respectively. The linear range of promethazine hydrochloride was 1-50 μg/mL, and the correlation coefficient was more than 0.9990. The recoveries of dioxopromethazine in different samples ranged from 95.9% to 104.8%. Conclusion The method is simple and accurate, and can be used for qualitative and quantitative detection of 3 kinds of antiasthmatic drugs illegally added in veterinary drugs and heathy food.