2018—2019年密云地区肠聚集性大肠埃希氏菌基因型及耐药分析

Genotype and drug resistance analysis of enteroaggregative Escherichia coli in Miyun district from 2018 to 2019

目的了解密云地区食源性疾患肠聚集性大肠埃希氏菌的感染情况、毒力基因型分布和耐药趋势。方法以密云区区医院、中医院为哨点医院,收集腹泻病人粪便868份。分离出68株肠聚集性大肠埃希氏菌,进行基因分型、耐药实验,并对结果进行统计学分析。结果 68株肠聚集性大肠埃希氏菌基因型aggR占51.47%,astA+pic基因占36.76%,aggR+astA+pic基因占11.76%,2019年比2018年肠聚集性大肠埃希氏菌氨苄西林耐药率升高21.88%(P<0.05),萘啶酸耐药率升高24.31%(P<0.05),四环素耐药率升高24.31%(P<0.05),其他8种抗生素耐药率变化无统计学意义(P>0.05)。结论建议继续积极主动监测食源性疾患病原,及时掌握本地区病原特征及耐药趋势,为肠聚集性大肠埃希氏菌感染的控制提供科学数据。

Objective To investigate the infection, genotype distribution and drug resistance trend of intestinal aggregation Escherichia coli in food borne diseases in Miyun district. Methods Taking Miyun district hospital and traditional Chinese medicine hospital as sentinel hospitals, 868 stool samples of diarrhea patients were collected according to the case definition. A total of 68 strains of enteroaggregative Escherichia coli were detected from feces. Genotyping and drug resistance tests were carried out, and the results were statistically analyzed. Results In 68 strains, aggR was take apart in 51.47%, astA+pic was 36.76% and aggR+astA+pic was 11.76%. In the drug sensitivity test of 11 kinds of antibiotics, the ampicillin resistance rate of E. coli in 2019 increased by 21.88%, compared with 201-8(P<0.05). The nalidixic acid resistance rate increased by 24.31%(P<0.05), and the tetracycline resistance rate increased by 24.31%(P<0.05). There was no significant change in the drug resistance rate of the other 8 kinds of antibiotics(P>0.05). Conclusion It is suggested to continue to monitor the pathogens of foodborne diseases actively, grasp the pathogen characteristics and drug resistance trend of pathogens timely in this area, and provide scientific data for the control of enteroaggregative Escherichia coli infection.