超高效液相色谱-串联质谱法测定植物源性食品中18种农药残留

Determination of 18 kinds of pesticide residues in plant-derived food by ultra performance liquid chromatography-tandem mass spectrometry

目的建立超高效液相色谱-串联质谱法检测植物源性食品中18种农药残留的方法。方法样品用乙腈溶解,经QuEChERS法萃取净化,离心后过滤膜,经超高效液相色谱-串联质谱分析,采用多反应监测模式测定,外标法定量。结果 18种农药残留在相应的浓度范围具有良好的线性关系(r≥0.993),各组分检出限为0.0003~0.003 mg/kg,定量限为0.001~0.01 mg/kg。18种农药残留加标回收率为63.3%~105.0%,所测定的相对标准偏差为0.86%~9.89%。结论该方法快速,准确,适用于测定大批量植物源性食品样品中的18种农药残留。

Objective To establish a method for rapid determination of 18 kinds pesticide residue in plant-derived food by ultra performance liquid chromatography-tandem mass spectrometry. Methods The samples were dissolved in acetonitrile, extracted and purified by QuEChERS method, centrifuged and filtered through a membrane. The samples were analyzed by ultra performance liquid chromatography-tandem mass spectrometry, determined by multi-reaction monitoring mode and quantified by external standard. Results The 18 pesticide residues had a good linear relationship in the corresponding concentration range(r≥0.993), the limits of detection of each component were 0.0003~0.003 mg/kg, and the limits of quantification were 0.001~0.01 mg/kg. The recoveries of 18 pesticide residues were 63.3%-105.0%, and the relative standard deviations were 0.86%-9.89%. Conclusion The method is fast, accurate, and suitable for the determination of 18 pesticide residues in large quantities of plant-derived food samples.