液相色谱-原子荧光光谱法测定8种中药材中砷和汞含量

Determination of arsenic and mercury in 8 kinds of Chinese medicinal materials by liquid chromatography-atomic fluorescence spectrometry

目的建立液相色谱-原子荧光光谱法(liquidchromatography-atomicfluorescencespectrometry,LC-AFS)检测山楂、甘草、白芍、西洋参、阿胶、金银花、枸杞子、黄芪8种药食同源中药材中砷和汞含量的方法。方法采用电热板消解、微波消解法、凯式消解和水浴消解4种不同前处理方法对样品进行处理,优化前处理技术,探索LC-AFS分析的最佳工作条件。结果砷的检出限为0.01mg/kg,汞的检出限为0.001 mg/kg,不同中药材中砷的回收率为82.4%~96.4%,汞的回收率为84.7%~95.5%,砷和汞的相对标准偏差为2.1%~5.6%。结论该方法快速、简便,准确度及灵敏度高,适用于中药材中的砷和汞的含量的测定。

Objective To establish a method for the determination of arsenic and mercury in 8 kinds of traditional Chinese medicine which are homology of medicine and food, including hawthorn, liquorice, Radix paeoniae Alba, American ginseng, donkey hide gelatin, honeysuckle, wolfberry and Astragalus by liquid chromatography-atomic fluorescence spectrometry(LC-AFS). Methods Four different pre-treatment methods including electric heating plate digestion, microwave digestion, Kjeldahl digestion and water bath digestion were used to treat the samples, and the pre-treatment technology was optimized to explore the best working conditions of LC-AFS analysis. Results The detection limits of arsenic and mercury were 0.01 mg/kg and 0.001 mg/kg, respectively. The recoveries of arsenic and mercury in different Chinese medicinal materials were 82.4%-96.4% and 84.7%-95.5%, respectively. The relative standard deviations of arsenic and mercury were 2.1%-5.6%. Conclusion The method is rapid, simple, accurate and sensitive, which is suitable for the determination of arsenic and mercury in traditional Chinese medicine.