蒸汽爆破与酶解耦合制备花生壳膳食纤维

Steam Explosion-Enzyme Extraction of Dietary Fiber from Peanut Shell

以花生壳为试验原料,研究了蒸汽爆破与酶解耦合(SEE)制备可溶性膳食纤维(SDF)工艺参数,并探讨了改性前后SDF的功能和结构特性。结果表明:最佳工艺参数为压力0.6 MPa,保压时间4 min,加酶量4.0%,酶解时间8 min,温度50℃,pH 4.0。在此条件下SDF提取率达到34.5%。Control-SDF和SEE-SDF的溶解性分别为82.14%和96.45%,持水力分别为1.89和3.47 g/g,持油力分别为1.19和2.31 g/g,膨胀力分别为2.98和5.17 mL/g;ABTS的清除率分别为54.52%和72.58%;·OH的清除率分别为35.18%和55.25%;O2-·的清除率分别为29.36%和61.09%;DPPH的清除率分别为41.24%和67.68%。改性后SDF结晶结构未受影响,特征衍射峰结晶面积更小,颗粒更致密,表面有明显的蜂窝状结构。研究结果可为花生壳膳食纤维的功能改性及综合利用提供理论依据。

With peanut shell as raw material,the process parameters of steam explosion-enzyme(SEE)extraction of SDF was researched and compared to the functional and structural properties.The result showed that the optimum condition was pressure 0.6 MPa,holding time 4 min,enzyme content 4.0%,time 8 min,temperature 50℃,pH 4.0.Under this condition,SDF extraction yield was 34.5%.The solubility of Control-SDF and SEE-SDF were 82.14%and 96.45%;Water holding capacity of Control-SDF and SEE-SDF were 1.89 and 3.47 g/g;Oil holding capacity of Control-SDF and SEE-SDF were 1.19 and 2.31 g/g;Swelling force of Control-SDF and SEE-SDF were 2.98 and 5.17 mL/g;The ABTS scavenging rates of ControlSDF and SEE-SDF were 54.52%and 72.58%;The·OH scavenging rates of Control-SDF and SEE-SDF were 35.18%and 5.25%;The O2-·scavenging rates of Control-SDF and SEE-SDF were 29.36%and 61.09%;The DPPH scavenging rates of Control-SDF and SEE-SDF were 41.24%and 67.68%.The SEE could not change the crystal structure and crystallization area was smaller of SDF.There was porous network like honeycomb and swelled structure of SEE-SDF.The research could provide a reference for functional modifications and utilization of dietary fiber from peanut shell.