microRNA-34a靶向调控血管内皮生长因子对U87脑胶质瘤细胞侵袭、增殖的影响

Effect of microRNA-34a on invasion and proliferation of U87 glioma cells via targeted regulation of VEGF

目的探究microRNA-34a靶向调控血管内皮生长因子(VEGF)对U87脑胶质瘤细胞侵袭、增殖的影响。方法将mi⁃croRNA-34a NC、microRNA-34a inhibtor、microRNA-34a mimic分别转染至U87脑胶质瘤细胞,依次设为NC组、miR-34a inhibtor组、miR-34a mimic组,比较三组脑胶质瘤细胞U87增殖、侵袭、迁移情况,荧光素活性,VEGFmRNA与蛋白表达情况。结果转染后,miR-34a mimic组细胞增值抑制率高于NC组和miR-34a inhibtor组(P<0.05);转染48 h后,miR-34a mimic组细胞侵袭、迁移能力低于NC组和miR-34a inhibtor组(P<0.05),miR-34a inhibtor组高于NC组(P<0.05);转染48 h后,miR-34a inhibtor组、miR-34a mimic组VEGFmRNA、蛋白相对水平分别高于、低于NC组[mRNA:(2.34±0.32)、(0.49±0.03)vs(1.00±0.06);蛋白:(2.42±0.16)、(0.43±0.06)vs(1.00±0.10)](F=256.230、83.750,均P<0.05)。结论microRNA-34a可以抑制脑胶质瘤细胞增殖、侵袭和迁移,其机制可能与抑制VEGF基因表达有关。

Objective Toexplore the effect of microRNA-34a on the invasion and proliferation of U87 glioma cells via targeted regulation of the vascular endothelial growth factor(VEGF).Methods U87 glioma cells transfected with microRNA-34a NC,microRNA-34a inhibitor,and microRNA-34a mimic were divided into NC group,miR-34a inhibitor group,and miR-34a mimic group,respectively.The three groups were compared for the proliferation,invasion,and migration of U87 glioma cells as well as fluorescein activity and the mRNA and protein expression of VEGF.Results After transfection,the cell proliferation inhibition rate in the miR-34a mimic group was significantly higher than that in the NC group and miR-34a inhibitor group(P<0.05);48 hours after transfection,the cell invasion and migration ability was significantly lower in the miR-34a mimic group than in the NC group and miR-34a inhibitor group(P<0.05),and was significantly higher in the miR-34a inhibitor group than in the NC group(P<0.05);48 hours after transfection,the relative mRNA and protein expression levels of VEGF were significantly higher in the miR-34a inhibitor group than in the NC group(mRNA:2.34±0.32 vs 1.00±0.06,protein:2.42±0.16 vs1.00±0.10,F=256.230,P<0.05),but the indices were significantly lower in the miR-34a mimic group than in the NC group(mRNA:0.49±0.03 vs 1.00±0.06,protein:0.43±0.06 vs 1.00±0.10,F=83.750,P<0.05).Conclusions MicroRN A-34a can inhibit the proliferation,invasion,and migration of glioma cells,which may be associated with the inhibition of VEGF gene expression.