水环境中胞内外抗生素抗性基因分析的DNA提取方法及污染现状研究进展

DNA Extraction Methods for Intracellular and Extracellular Antibiotic Resistance Genes and Their Pollution Status in Aquatic Environment

新型污染物抗生素抗性基因(Antibiotic resistance genes,ARGs)对公共健康产生了严重威胁,其在水环境中的长期存在和传播引起了广泛重视。胞内与胞外ARGs在水环境中的分布规律和环境行为差异明显,对其污染状况进行研究有助于全面了解水环境中ARGs的传播特性。该文总结了5种常见水样微生物DNA提取方法及其在胞内和胞外ARGs检测研究中的应用状况,比较了污水系统、淡水系统及海水系统等典型水环境中胞内和胞外ARGs的污染状况和迁移传播特性。为进一步探究水环境中ARGs的分布特征与传播机制提供了技术手段及污染现状等基础信息,对控制ARGs在水环境中的传播扩散具有重要意义。

Antibiotic resistance genes(ARGs)may cause serious threat to public health,which are regarded as an emerging contaminant.Their presence and propagation in aquatic environment have attracted great attention.Increasing studies show that intracellular ARGs and extracellular ARGs exhibit different distribution patterns and environmental behaviours.It is necessary to investigate their pollution statuses for a comprehensive understanding of ARGs propagation in aquatic environment.The mechanisms of five common microbial DNA extraction methods and their applications in the detection of intracellular and extracellular ARGs in water samples are summarized in this study.The contamination status and migration propagation characteristics of intracellular and extracellular ARGs in three typical aquatic environments,i.e.sewage system,surface fresh water system and marine system are compared.The basic information regarding the technological means and pollution status of ARGs pollution in aquatic environment is provided in this review,which is of great significance for the control of ARGs in aquatic environment.