海参花成分分析及对小鼠认知辨别能力的影响

Composition Analysis of Sea Cucumber Ovum and Its Effect on Cognitive Discrimination of Mice

本研究主要对海参花进行了成分分析并研究了其对小鼠认知辨别能力的影响。70只SPF级雄性ICR小鼠按体重随机分为空白组、东莨菪碱模型组、吡拉西坦阳性组(400 mg/kg),海参花低、中、高剂量组(75、150、450 mg/kg)。每天灌胃给药30 min后腹腔注射1 mg/kg东莨菪碱造模。30 d后进行旷场实验、新物体识别实验、Y迷宫、Morris水迷宫、避暗实验等行为学实验。在行为学实验训练期继续灌胃给药并造模。结果表明:与模型组相比,海参花组能显著增加小鼠在旷场中心区域活动的时间(P<0.05),在新物体识别实验中能显著提高小鼠的辨别指数(P<0.05),在Y迷宫实验中能显著增加自发交替(P<0.01),在Morris水迷宫空间探索实验中能显著减少小鼠的逃避潜伏期(P<0.01),同时显著增加小鼠在平台所在区域的穿越次数(P<0.05)和累计时间(P<0.05)。在避暗实验测试期中能显著增加小鼠进入暗室的潜伏期(P<0.05)和降低错误次数(P<0.05)。

This study mainly analyzed the components of sea cucumber ovum and studied their effects on the cognitive discrimination in mice.70 SPF(Specific Pathogen Free)male ICR(Institute of Cancer Research)mice were randomly divided into the control group,scopolamine group,piracetam positive group(400 mg/kg),sea cucumber ovum low,medium and high dose groups(75,150 and 450 mg/kg).Mice were intragastrically administered 30 minutes before intraperitoneal injection of 1 mg/kg scopolamine daily.After 30 days,mice were subjected to different behavioral experiments including open field test,new object recognition test,Y maze,Morris water maze,and step-through task.During the behavioral experimental training period,oral administration and scopolamine injection were continued to establish the model.The results showed that compared with the scopolamine group,the sea cucumber ovum group significantly increased the active time of mice in the central area of open field(P<0.05),and significantly improved their discrimination index in the object recognition test(P<0.05).Spontaneous alternation was significantly increased in the Y maze(P<0.01),and the escape latency was significantly reduced in the Morris water maze space exploration test(P<0.01).At the same time,it significantly increased the crossing times(P<0.05)and cumulative time(P<0.05)of mice in the platform area.During the dark avoidance experiment,the latency of mice entering the dark room was significantly increased(P<0.05)and the number of errors was significantly decreased(P<0.05).