石鸡十二指肠嗜银细胞的分布及形态观察

Distribution and Morphology Observation of Argyrophil Cells in Duodenum of Chukar Partridge(Alectoris chukar)

石鸡是具有丰富营养价值的特种经济动物,人工养殖越来越多,目前石鸡组织学和细胞学方面的研究资料十分有限。为了解石鸡十二指肠结构和嗜银细胞的分布特点,通过石蜡组织切片和Grimelius嗜银染色方法,对十二指肠管壁内嗜银细胞的形态特点及其分布规律进行研究。结果表明,十二指肠管壁内嗜银细胞呈棕黑色,主要分布于肠绒毛和小肠腺。十二指肠绒毛中的嗜银细胞位于固有层中,多呈长条形或纺锥形,靠近中央乳糜管;小肠腺嗜银细胞较多,多数位于腺上皮细胞之间或基底部,呈纺锤形、锥形或近圆形,常见细胞突起伸向小肠腺管腔。十二指肠嗜银细胞在小肠腺的分布密度大于绒毛处,且封闭型嗜银细胞数量多于开放型嗜银细胞。

The chukar partridge(Alectoris chukar)is a kind of special economic fowl with rich nutritional value.At present,more and more chukar partridges are farmed,but there are few studies on their histology and cytology.In order to understand the structures and distribution characteristics of special cell types in the duodenum of chukar partridges,the paraffin sections and Grimelius histochemistry staining method were employed to identify and count the argyrophil cells in the duodenal wall of chukar partridges.The argyrophil cells with black cytoplasm and negative nuclei locate in the lamina propria of villi and small intestine glands in the duodenal wall.The argyrophil cells in the lamina propria of villi are long and spindle shaped,and they are close to the central chyloid ducts.The argyrophil cells in the small intestine glands locate between the glandular epithelial cells and at the base,and appear as fusiform,conical and suborbicular.Their cytoplasmic processeses were observed frequently extending into the lumen of small intestine glands.The distribution density of the argyrophil cells in the small intestine glands is much greater than that in the villi.Moreover,the number of open type argyrophil cells is more than that of closed type.