LINC01106靶向miR-744-5p影响乳腺癌MDA-MB-231细胞增殖、迁移及侵袭

LINC01106 Affects the Proliferation,Migration and Invasion of Breast Cancer MDA-MB-231 Cells by Targeting MiR-744-5p

目的探讨LINC01106对乳腺癌细胞增殖、迁移和侵袭的影响及可能机制。方法收集39例乳腺癌组织及癌旁组织,RT-qPCR法检测组织中LINC01106和miR-744-5p表达水平。体外培养乳腺癌细胞MDA-MB-231,分别转染si-LINC01106、miR-744-5p模拟物、或共转染si-LINC01106与anti-miR-744-5p,采用MTT法和克隆形成实验检测细胞增殖,划痕实验和Transwell分别检测细胞迁移和侵袭,免疫印迹法检测细胞中E-cadherin和N-cadherin蛋白表达。双荧光素酶报告基因实验验证LINC01106和miR-744-5p调控关系。结果乳腺癌组织中LINC01106的表达高于癌旁组织(P<0.05),miR-744-5p的表达低于癌旁组织(P<0.05)。干扰LINC01106或过表达miR-744-5p可降低MDA-MB-231细胞OD值、克隆形成数、划痕愈合率、侵袭数及细胞中N-cadherin蛋白表达(P<0.05),而促进了E-cadherin蛋白表达(P<0.05)。LINC01106靶向负调控miR-744-5p,干扰miR-744-5p可逆转干扰LINC01106对MDA-MB-231细胞增殖、迁移和侵袭的抑制作用。结论LINC01106在乳腺癌组织中表达升高,其可能通过靶向负调控miR-744-5p促进乳腺癌细胞的增殖、迁移和侵袭,有可能成为乳腺癌治疗的新分子靶点。

Objective To investigate the effect of LINC01106 on the proliferation,migration and invasion of breast cancer cells and its potential mechanism.Methods 39 breast cancer tissues and adjacent tissues were collected for the study,and the expression levels of LINC01106 and miR-744-5p in the tissues were detected by RT-qPCR.Breast cancer cells MDA-MB-231 were cultured in vitro and transfected with si-LINC01106 or miR-744-5p mimic,or co-transfected with si-LINC01106 and anti-miR-744-5p,respectively.Cell proliferation was detected by MTT method and clone formation experiment,and cell migration and invasion were detected by scratch test and Transwell assay.The protein expressions of E-cadherin and N-cadherin in cells were detected by western blotting.The dual luciferase reporter gene experiment was conducted to verify the regulatory relationship between LINC01106 and miR-744-5p.Results The expression of LINC01106 in breast cancer tissue was higher than that of adjacent tissues(P<0.05),while the expression of miR-744-5p was lower than that of adjacent tissues(P<0.05).The interference LINC01106 expression or overexpression of miR-744-5p reduced the OD value of MDA-MB-231 cells,the number of clone formation,scratch healing rate,the number of invasion and the protein expression of N-cadherin(P<0.05),while promoted the protein expression of E-cadherin(P<0.05).LINC01106 negatively regulated the expression of miR-744-5p.Interfering with miR-744-5p reversed the inhibitory effect of interfering with LINC01106 on the proliferation,migration and invasion of MDA-MB-231 cells.Conclusion The expression of LINC01106 is elevated in breast cancer tissues,and it may promote the proliferation,migration and invasion of breast cancer cells by negatively regulating the expression of miR-744-5p,which could become a new molecular target for breast cancer treatment.