流动注射化学发光法研究桔子皮提取液的抗氧化性

Study on Antioxidant Activity of Crude Extract of Orange Peel by Flow Injection Chemiluminescence

以桔子皮为原料,研究提取桔子皮活性物质的最佳工艺及其抗氧化性。采取超声辅助法对桔子皮中消除超氧阴离子的活性物质进行提取,通过单因素实验对提取工艺进行了优化,利用Luminol-邻苯三酚-Na_(2)CO_(3)化学发光体系评价了桔子皮提取液消除超氧阴离子的能力。结果表明,在温度为60℃、80%的乙醇为提取剂、料液比为1∶20、超声30 min提取5 h条件下,提取液消除超氧阴离子的能力达到最高。

To study the best extracting process and antioxidant of active substances from orange peel.Ultrasonic assisted method was used to extract the active substances from orange peel to eliminate superoxide anions.The extraction process was optimized by single factor experiment.The ability of orange peel extract to eliminate superoxide anions was evaluated by Luminol-pyrogallol Na_(2)CO_(3) chemiluminescence system.The results showed that the optimized conditions were determined as follows:extraction solvent 80% ethanol,solid-solvent ratio 1∶20(g/mL),ultrasonic treatment time 30 min,extraction time 5 h extraction temperature 60℃;Under these conditions,the rate of cleaning superoxide anion was the best.