人乳牙牙髓干细胞治疗肝硬化小鼠模型的研究

A study on the therapeutic effect of stem cells from human exfoliated deciduous teeth on a mouse model of liver cirrhosis

目的研究人乳牙牙髓干细胞(SHED)治疗肝硬化小鼠的效果。方法建立CCl4诱导的肝硬化模型,治疗组和对照组小鼠分别经尾静脉注射SHED和0.9%NaCl溶液,4周后评价肝功能指标及肝脏病理学表现。结果SHED治疗组与对照组小鼠总胆红素为(4.73±0.06)μmol/L比(6.27±0.50)μmol/L、ALT为(48.33±5.13)U/L比(64.33±3.06)U/L、AST为(107.67±17.56)U/L比(149.00±7.55)U/L和碱性磷酸酶为(142.00±28.93)mmol/L比(240.00±30.51)mmol/L,均较对照组显著好转。治疗组小鼠的体质量为(25.80±1.02)g比(22.86±0.97)g、肝脏湿重为(2.16±0.11)g比(1.88±0.15)g均较对照组显著增加,差异均有统计学意义(t=3.347、4.668,均P<0.05)。SHED治疗组小鼠肝脏组织纤维化及炎症浸润程度较对照组明显改善。结论在肝硬化小鼠模型中,经SHED治疗组小鼠的肝功能及肝脏病理学表现较对照组显著改善。提示SHED对肝硬化具有治疗作用。

Objective To evaluate the therapeutic effect of stem cells from human exfoliated deciduous teeth(SHED)on a mouse model of liver cirrhosis.Methods A mouse model of experimental liver cirrhosis was induced by introperitoneally injection of CCl4.SHED and normal saline solution were injected through the tail veins in the experimental group and the control group per week,respectively.After 4 weeks injection,liver function and liver pathological manifestations of the mice were evaluated.Results The total bilirubin(4.73±0.06μmol/L vs 6.27±0.50μmol/L,P=0.038),alanine aminotransferase(48.33±5.13 U/L vs 64.33±3.06 U/L,P=0.0097),aspartate aminotransferase(107.67±17.56 U/L vs 149.00±7.55 U/L,P=0.020)and alkaline phosphatase(142.00±28.93 mmol/L vs 240.00±30.51 mmol/L,P=0.0156)of the SHED group were significantly recovered than those of the control group.The body weight(25.80±1.02 g vs 22.86±0.97 g,P=0.0016)and liver weight(2.16±0.11 g vs 1.88±0.15 g,P=0.0101)of the SHED group regained as well.Meanwhile,the pathological manifestation of liver tissues after SHED treatment was obviously reversed in mice of the SHED group.Conclusion In CCl4 induced mouse model of liver cirrhosis,both the liver functions and pathology manifestations were significantly improved after SHED treatment,which indicated that SHED had a promising therapeutic effect on liver cirrhosis.