Rspo1与EMT相关蛋白在胃癌中的表达及其临床意义

Expression of Rspo1 and Epithelial-mesenchymal Transition-related Proteins in Gastric Cancer and Its Clinical Significance

[目的]探讨特异性顶部盘状底板反应蛋白1(Rspo1)与上皮间质转化(epithelialmesenchymal transition,EMT)相关蛋白波形蛋白(Vimentin)和E-钙黏蛋白(E-cadherin)在胃癌中的表达水平及其临床意义。[方法]通过免疫组化SP法测定115例胃癌组织及20例癌旁正常组织标本中Rspo1和Vimentin、E-cadherin的表达,分析其与临床病理特征的关系,Rspo1和EMT相关蛋白之间的相关性以及与胃癌患者预后的关系。[结果]胃癌组织中Rspo1和Vimentin阳性表达率(54.8%和34.8%)明显高于癌旁正常组织(25.0%和0)(P均<0.05),而胃癌组织中E-cadherin阳性表达率(53.9%)明显低于癌旁正常组织(100%)(P<0.05)。胃癌组织中Rspo1表达与肿瘤大小、TNM分期、浸润深度、淋巴结转移及远处转移相关(P均<0.05),与患者性别、年龄、分化程度无关。Rspo1与Vimentin的表达呈正相关(r=0.333,P<0.05),与E-cadherin的表达呈负相关(r=-0.349,P<0.05)。Rspo1和Vimentin高表达患者生存率更低(P<0.05),E-cadherin阴性组患者较阳性组患者生存率更低(P<0.05)。多因素Cox回归分析显示:TNM分期Ⅲ~Ⅳ期、淋巴结转移、远处转移、Rspo1表达阳性及E-cadherin表达阴性是影响胃癌患者预后的独立危险因素(P<0.05)。[结论]Rspo1在胃癌组织中高表达,且与EMT相关蛋白的表达密切相关,Rspo1可能通过诱导EMT的发生促进胃癌的侵袭和转移,进而影响患者预后。

[Objective]To investigate the expression and clinical significance of roof plate specific spondin 1(Rspo1)and epithelial-mesenchymal transition(EMT)-related proteins Vimentin and Ecadherin in gastric cancer.[Methods]Immunohistochemical staining was performed to determine the expression levels of Rspo1,Vimentin and E-cadherin in 115 samples of gastric cancer tissue and 20 samples of paracancerous tissue.Relationship between survival rates and clinicopathological features of the patients with gastric cancer,and correlation of Rspo1 with E-cadherin and Vimentin,were analyzed.[Results]The positive rate of Rspo1 expression and Vimentin in gastric cancer tissue(54.8%and 34.8%)were significantly higher than those in paracancerous tissues(25.0%and 0)(all P<0.05),and the positive rate of E-cadherin in gastric cancer tissue(53.9%)was significantly lower than that in paracancerous tissues(100%)(P<0.05).The high expression of Rspo1 in tumor tissues was closely related to tumor size,TNM stage,depth of invasion,lymph node metastasis,and distant metastasis(all P<0.05),but was not associated with gender,age,and tumor differentiation.The expression of Rspo1 in gastric cancer tissues was negatively correlated with E-cadherin(r=-0.349,P<0.05)and positively correlated with Vimentin(r=0.333,P<0.05).Patients with high expression levels of Rspo1 and Vimentin had significantly poorer prognosis and lower survival rate than those with low expression(P<0.05),whereas patients with low expression levels of E-cadherin had significantly poorer prognosis and lower survival rate than those with high expression(P<0.05).Cox regression analysis showed that TNM stageⅢ~Ⅳstage,lymph node metastasis,distant metastasis,positive Rspo1 expression,and negative E-cadherin expression were the independent prognostic factors of gastric cancer(P<0.05).[Conclusion]Rspo1 is highly expressed in gastric cancer tissues,which is significantly associated with EMT-related proteins(Ecadherin,Vimentin).Rspo1 may promote the invasion and metastasis of gastric cancer by inducing the occurrence of EMT,thereby affecting the prognosis of patients.