不同负压吸引装置对小鼠皮肤分泌胶原蛋白和基质金属蛋白酶1及转化生长因子-β的影响

Effects of different negative pressure suction devices on collagen,MMP-1 and TGF-βsecretion of skin

目的比较圆拱形装置和敷料型装置对吸引区皮肤的胶原蛋白、基质金属蛋白酶(MMP-1)、转化生长因子-β(TGF-β)表达的影响,探讨受吸引区皮肤的改变方式及机制.方法2018年3-5月,在广州南方医院动物实验中心用已建立的动物模型,将54只小鼠均分为3组:对照组、圆拱形装置组、敷料装置组.分别在负压吸引处理后1、3、5d取材,在皮肤组织HE切片上直接测量比较皮肤真皮层厚度,用分光光度法比较局部皮肤组织胶原蛋白含量,用ELISA法比较局部皮肤组织中MMP-1和TGF-β表达量.结果圆拱形装置组与敷料装置组均能增加真皮层厚度,差异有统计学意义[处理5d时,对照组:(71±8)μm;圆拱形装置组:(351±9)μm;敷料装置组:(267±12)μm,P<0.05].圆拱形装置组和对照组相比,真皮层中胶原蛋白含量显著升高,差异有统计学意义[处理5d时,对照组:(30.9±4.3)mg/g;圆拱形装置组:(72.7±3.6)mg/g,P<0.05].圆拱形装置组的TGF-β含量呈现逐渐增加趋势,比敷料装置组和对照组显著增加(处理5d时,对照组:[0.24±0.1)ng/ml;圆拱形装置组:(0.78±0.08)ng/ml;敷料装置组:(0.39±0.18)ng/ml,P<0.05].而MMP-1分泌则呈现为逐渐减少趋势,敷料装置组TGF-β和MMP-1分泌在全实验过程中变化不大.结论圆拱形负压吸引装置界面通过造成成纤维细胞空间形变,更有效激活成纤维细胞,促进TGF-β和胶原蛋白分泌,增厚皮肤组织.

Objective To compare the effects of circular arch device and dressing device on the secretion of collagen,matrix metalloproteinase(MMP-1),transforming growth factor(TGF-β)of fibroblasts in the treated skin,and to explore the changes and mechanisms.Methods The animals were purchased from the Southern Medical University Laboratory Animal Center.Using the established animal model,54 mice were randomly divided into three groups:18 mice each group,including control group,dressing device group and and circular arch device group.At the first,third and fifth day after negative pressure suction treatment,dermal thickness and density of collagen levels were and eosin staining,collagen level in local skin was determined using spectrophotometry,and MMP-1 and TGF-p levels were determined using enzyme linked immunosorbent assay.Results Dermal thickness level was increased significantly in negative suction treated groups[after 5 days of treatment,control group,(71±8)jim;circular arch device group:(351±9)μm;dressing device group:(267±12)μm,P<0.05],Compared with the control group,collagen level in the dermis was significantly higher in circular arch device groups[after 5 days of treatment,control group:(30.9+4.3)mg/g;circular arch device group:(72.7±3.6)mg/g,P<0.05],TGF-βlevel in circular arch device treated group was increasing gradually,and markedly higher than that in the dressing device group and the control group[after 5 days of treatment,control group;(0.24±0.1)ng/ml;circular arch device group,(0.78±0.08)ng/ml;dressing device group,(0.39±0.18)ng/ml,P<0.05],while MMP-1 level was decreasing.In addition,TGF-βand MMP-1 levels in the dressing device treated group remained stable during the entire experiment.Conclusions The interface of the circular arch negative pressure suction device can effectively activate the fibroblasts,promote the secretion of TGF-βand collagen,and thicken the skin tissue.