摘要
半导体制冷片(TEC)的布置间距是影响聚合酶链式反应(PCR)仪温度均匀性的重要因素之一。本文分析了PCR仪热循环系统的基本结构及传热原理,针对某型号PCR仪构建了三维传热模型,并运用数值仿真方法研究了TEC布置间距对PCR仪温度均匀性的影响规律。结果表明,TEC的布置间距越大,PCR反应试液的温度均匀性越好。
The gap between thermal electric coolers(TEC)is an essential factor affecting the temperature uniformity of polymerase chain reaction(PCR)instrument.In this paper,the structure and heat transfer mechanism of the PCR instrument was investigated,and a three-dimensional heat transfer mathematical model for a specific type of PCR instrument was modeled.The finite element analysis method was adopted to study the effect of TEC gap on temperature uniformity of PCR instrument.The results showed that the temperature uniformity of PCR test solution became better with the increasing of TEC gap.
作者
孙继贤
张辰
魏名山
祝天宇
田冉
李征
SUN Jixian;ZHANG Chen;WEI Mingshan;ZHU Tianyu;TIAN Ran;LI Zheng
出处
《计量与测试技术》
2021年第5期1-4,共4页
Metrology & Measurement Technique
基金
装备发展部技术项目,聚合酶链反应分析仪温度场自动校准装置的研制及校准方法的建立(项目编号:192JK22004)。
关键词
PCR仪
TEC间距
温度均匀性
传热机理
有限元方法
PCR instrument
TEC gap
temperature uniformity
heat transfer mechanism
finite element methods
