益气活血方对肝纤维化大鼠JNK通路的作用研究

Effect of Yiqi Huoxue Recipe on JNK Pathway in Rats with Hepatic Fibrosis

目的:研究c-Jun氨基末端激酶(JNK)信号转导通路在CCl 4诱导的肝纤维化大鼠模型中的表达,以阐明益气活血方抑制肝纤维化的分子机制。方法:27只雄性Wistar大鼠随机分为对照组、模型组和益气活血方治疗组。模型组以30%CCl 4橄榄油溶液腹腔注射诱导建立肝纤维化模型;益气活血方治疗组在CCl 4建模的同时以益气活血方灌胃进行干预实验;对照组腹腔注射橄榄油溶液。检测大鼠血清丙氨酸氨基转移酶(ALT)及天冬氨酸氨基转移酶(AST)水平;分别采用RT-PCR及Western Blot法检测大鼠肝组织JNK信号通路中JNK、c-Jun mRNA表达及其磷酸化产物p-JNK、p-c-Jun蛋白表达。结果:模型组大鼠血清ALT、AST水平、肝组织JNK、c-Jun mRNA及p-JNK、p-c-Jun蛋白表达增高,与对照组相比差异有显著性,而益气活血方治疗组可显著降低ALT、AST、c-Jun mRNA及p-JNK、p-c-Jun蛋白表达水平(P均<0.05)。结论:益气活血方通过抑制JNK信号转导通路的活化,从而延缓肝纤维化进展。

To study the expression of c-Jun N-terminal kinase(JNK)signal transduction pathway in CCl 4-induced liver fibrosis rat model,and to elucidate the molecular mechanism of Yiqi Huoxue Recipe in inhibiting liver fibrosis.Methods:27 male Wistar rats were randomly divided into control group,model group and Yiqi Huoxue Recipe treatment group.The model group was induced by intraperitoneal injection of 30%CCl 4 olive oil solution;the treatment group was given Yiqi Huoxue Decoction by gavage while CCl 4 model was established;the control group was intraperitoneally injected with olive oil solution.Serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)levels were detected;RT-PCR and Western Blot were used to detect the mRNA expression of JNK and c-Jun and the protein expression of p-JNK and p-c-Jun.Results:The levels of ALT and AST in serum,the expressions of JNK,c-Jun mRNA and p-JNK,p-c-Jun protein in liver tissue of the model group were significantly higher than those of the control group,while those in Yiqi Huoxue Decoction treatment group were significantly lower than those of the control group(P<0.05).Conclusion:Yiqi Huoxue Recipe can delay the progression of liver fibrosis by inhibiting the activation of JNK signal transduction pathway.