产絮凝剂菌株Pseudoalteromonas undina JP的表型特征及基因组学分析

Phenotypic Characterization and Genomic Analysis of Flocculant-Producing Bacteria Pseudoalteromonas undina JP

从菲律宾蛤仔黏附污泥中分离到1株絮凝率高达80.0%的高絮凝活性菌株JP,结合其序列比对和生理生化指标结果,菌株JP被鉴定为Pseudoalteromonas undina。通过Illumina测序平台对菌株JP进行基因组框架序列扫描测序,获得20个总长度为4046116 bp的框架序列,在这些框架序列中共预测到14个rRNA、87个tRNA和3746个基因,注释结果表明其主要参与肽聚糖、脂多糖、氨基糖、核苷酸糖、淀粉、蔗糖、果糖、甘露糖的生物代谢。产胞外多糖类絮凝剂代谢途径中关键前体、重复寡糖单元和调控基因均有发现,虽未找到eps基因簇,推测其可能具有与eps基因簇相似的基因承担对应的功能。

Ruditapes philippinarum conglutination mud(RPM)was reported as a promising natural bioflocculant resource,whose flocculating active substances may be produced by its symbiotic microorganism.A strain JP,isolated from RPM with 80.0%of flocculation rate,were identified as Pseudoalteromonas undina according to the results of biochemical tests and blast.The scaffold sequences of complete genome were carried out using Illumina sequencing platform.The genome contained 20 scaffolds with 4046116 bp encoding 3746 genes,14 rRNA and 87 tRNA in total.The bioinformatic analysis showed that the genome of P.undina JP was related to starch,sucrose,fructose and mannose uptake system,and metabolism of peptidoglycan,lipopolysaccharide,amino sugar and nucleotide sugar.The key precursors,repetitive oligosaccharide units(including UDP-ManNAcA、dNTP-D-Glu、UDP-3-O-(3-acetyl)-GlcNAc)and regulatory genes in the extracellular polysaccharide flocculant metabolic pathway were found.However,the eps gene cluster was not found in this study,it may have a function similar to the gene of the eps gene cluster because the strain JP had flocculation activity.