“腰楼大葱”组织培养技术及田间对比研究

Tissue Culture Technique and Field Comparative Study of‘Yaolou scallion(Allium fistulosum)’

[目的]筛选最佳浓度NAA“腰楼大葱”生根培养基,比较组培苗和非组培苗的优劣。[方法]以“腰楼大葱”茎尖为外植体材料,接种到不同浓度NAA生根培养基上,将获得的组培苗与非组培苗进行田间对比试验。[结果]生根壮苗最佳NAA浓度为0.10 mg/L;组培苗与非组培苗的直立叶数和老叶个数均存在显著差异;非组培苗的平均倒伏叶数为6.40片,组培苗仅有3.29片,且存在极显著差异。[结论]“腰楼大葱”组培苗商品性明显优于非组培苗。

[Objective]To select the best concentration of NAA‘Yaolou scallion’rooting medium,the advantages and disadvantages of tissue culture and non-tissue culture seedlings were studied.[Method]Shoot tips of‘Yaolou scallion’were used as explants and cultured on different concentrations of NAA rooting medium.The tissue culture seedlings and non-tissue culture seedlings were compared in the field.[Result]The results showed that the optimum concentration of NAA was 0.10 mg/L,the number of erect leaves and the number of old leaves were significantly different between tissue-cultured and non-tissue-cultured plantlets,the average number of lodging leaves of non-tissue-cultured plantlets was 6.40,and that of tissue-cultured plantlets was only 3.29.There was a significant difference in the average number of lodging leaves between non-tissue culture seedlings and tissue culture seedlings.[Conclusion]The commercial quality of tissue-cultured plantlets was better than that of non-tissue-cultured plantlets.