切花红掌组培繁殖技术研究

Study on in vitro Propagation of Anthurium andraeanum‘Floris’

[目的]建立切花红掌组织培养快繁体系。[方法]选用切花红掌品种“佛莱”为试材,研究不同外植体、激素对其组织培养诱导、分化和生根的影响。[结果]适宜红掌“佛莱”建立无菌系的外植体为4月10日的顶芽组织;初代诱导培养基为MS+6-BA 1.0 mg/L+NAA 0.3 mg/L;继代培养基为MS+6-BA 0.8 mg/L+NAA 0.3 mg/L,增殖系数达19.4倍;生根培养基为1/2MS+IBA 0.5 mg/L,生根率100%。[结论]该研究建立的红掌“佛莱”组织培养快繁体系可为切花红掌工厂化生产提供技术参考。

[Objective]The research aimed to establish a rapid prapagation system of Anthurium andraeanum.[Method]Taking Anthurium andraeanum‘Floris’as test material,the effects of different explants and hormones on the induction,differentiation and rooting of tissue culture were studied.[Result]The suitable explants for establishing aseptic line of Anthurium andraeanum‘Floris’were the apical bud tissue on April 10,the initial induction medium was MS+6-BA 1.0 mg/L+NAA 0.3 mg/L,the medium of subculture micropropagation was MS+6-BA 0.8 mg/L+NAA 0.3 mg/L on which reproductive rate reached 19.4,and 1/2MS+IBA0.5 mg/L was the rooting medium on which rooting rate reached 100.00%.[Conclusion]The suitable tissue culture formula of Anthurium andraeanum‘Floris’was selected,which can provide the technical support for large scale breeding.