驴胎盘蛋白的组成分析

Analysis of the Composition in Protein from Donkey Placenta

采用蛋白质传统分离技术结合基质辅助激光解吸电离飞行时间质谱(MALDI-TOF/TOF/MS)揭示驴胎盘蛋白质的性质和组成,结果表明,驴胎盘蛋白含量为(15.62±1.04)%,等电点均匀分布酸性和碱性区域,分子量主要集中在7~19、36~78 kD 2个区域。通过与马科蛋白图库进行对比,检索出3865种可识别的蛋白,进一步对驴胎盘蛋白进行生物信息学分析,发现驴胎盘蛋白主要参与了内质网蛋白质加工、ECM-受体相互作用、内质网应激反应等生物学过程,为驴胎盘药理活性成分的筛选提供了理论基础。

SDS-PAGE gel electrophoresis and MALDI-TOF/TOF/MS were used to reveal the properties and composition of protein in donkey placenta.The results showed that the protein content of the donkey placenta was(15.62±1.04)%,the isoelectric point was evenly distributed in the acidic and basic regions,and the molecular weight was mainly concentrated in the two regions of 7-19 and 36-78 kD.By comparing with the equine protein library,3865 identifiable proteins were retrieved,and further analysis of donkey placental proteins was performed.It was found that donkey placental proteins were mainly involved in biological processes such as protein processing in endoplasmic reticulum,ECM-receptor interaction and response to endoplasmic reticulum stress.It provided a theoretical basis for the screening of donkey placenta pharmacologically active ingredients.