一测多评法测定甘肃地区淫羊藿黄酮类成分的含量

Determination of Flavonoids of Epimedium in Gansu by Quantitative Analysis of Multi-components with a Single Marker(QAMS)

[目的]建立一测多评法同时测定淫羊藿中6种黄酮类成分的方法,并对甘肃地区的21批药材进行定量分析。[方法]Agilent Zorbax色谱柱(4.6 mm×250 mm,5μm),流动相为水(A)、乙腈(B),梯度洗脱,流速0.8 mL/min,检测波长270 nm,进样量10μL。分别考察不同条件对相对校正因子的影响。评价一测多评法和标准曲线法在含量测定结果上的差异。[结果]建立了以淫羊藿苷为内标参照物,同时测定6种黄酮类成分的一测多评法。不同条件下相对校正因子的RSD小于2.63%。多点矫正-一测多评法、斜率矫正-一测多评法和标准曲线法测定21批甘肃地区淫羊藿样品含量,其RSD小于3%,无明显差异。[结论]一测多评法结果准确、操作简单易行,可用于淫羊藿中黄酮类成分的含量测定。

[Objective]To establish a method for simultaneous determination of 6 flavonoids in Epimedium by QAMS,and to determine the content of 21 batches of medicinal materials in Gansu Province.[Method]Agilent Zorbax column(4.6 mm×250 mm,5μm),mobile phase were water(A)and acetonitrile(B),gradient elution,flow rate was 0.8 mL/min,the detection wavelength was set at 270 nm,injection volume 10μL.The effects of different conditions on relative correction factors were investigated.The difference between the standard curve method and QAMS was evaluated in content determination.[Result]A method was established to determine 6 flavonoids by using icariin as internal reference based on QAMS.The RSD under different conditions was less than 2.63%.The content of 21 batches of Epimedium in Gansu Province was determined by QAMS and standard curve method,the RSD of two sets was less than 3%and there was no significant difference.[Conclusion]QAMS is accurate,easy to operate,and can be used to determine the content of flavonoids in Epimedium.